Aryl hydrocarbon receptor ligand activity of commercial health foods
Aryl hydrocarbon receptor ligand activity of commercial health foods
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DOI：
10.1016/j.foodchem.2010.12.034
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发表时间：
2011-06-15
期刊：
影响因子：
8.8
通讯作者：
Yoshida, Takashi
中科院分区：
文献类型：
--
作者：
Amakura, Yoshiaki;Tsutsumi, Tomoaki;Yoshida, Takashi
芳烃受体（AhR）是一种配体激活的转录因子，通过与二恶英等激动剂结合介导毒理学效应。我们以前报道过食品中存在天然的类二恶英配体。为了进一步表征具有二恶英样活性的天然配体，我们使用报告基因测定法检查了50种商业补充剂和保健食品对AhR的影响。一些以大豆、芝麻或蜂胶为原料制备的样品显示出ahr结合活性，类似于高浓度的2,3,7,8-四氯二苯并-对二恶英（TCDD）。为了表征8个活性样品中的AhR活性物质，分别用正己烷、乙酸乙酯和水对提取物进行分馏，然后估计其AhR活性。蜂胶提取物样品的正己烷部分和其他样品的乙酸乙酯部分在高浓度范围内表现出与TCDD相似的AhR活性。高效液相色谱法分析发现，样品中含有大豆苷元、糖苷元等异黄酮，以及菊花素、菊花素等黄酮类化合物。在这些化合物中，鸢尾菊素表现出明显的AhR激活。黄酮类化合物的特征是天然AhR配体，已知对人体健康具有代表性的有益作用。本研究中鉴定的天然AhR配体已知对人类健康有益。因此，我们认为AhR可能在人类中发挥有益的调节作用。(C) 2010 Elsevier Ltd.版权所有。
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates toxicological effects by binding to agonists such as dioxins. We previously reported the presence of natural dioxin-like ligands in foods. To further characterise natural ligands with dioxin-like activity, we examined the influence of 50 kinds of commercial supplement and health food on the AhR, using a reporter gene assay. Some samples, prepared using soybean, sesame, or propolis as an ingredient, were revealed to show AhR-binding activity, similar to that of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), at high concentrations. To characterise the AhR-activating substances in eight active samples, the respective extracts were subjected to fractionation with n-hexane, ethyl acetate, and water, followed by estimating their AhR activities. The n-hexane fraction of the propolis extract sample, and the ethyl acetate fractions of the other samples, showed AhR activity similar to that of TCDD, at a high concentration range. HPLC analysis of the active fractions identified isoflavones, such as daidzein and glycitein, and flavones, such as tectochrysin and chrysin, in the samples. Among these compounds, tectochrysin exhibited marked AhR activation. Flavonoids, which are characterised as natural AhR ligands, are known to have representative beneficial effects on human health. The natural AhR ligands identified in this study are known to be useful for human health. Therefore, it is considered that AhR may play a beneficial regulatory role in humans. (C) 2010 Elsevier Ltd. All rights reserved.