USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma.
USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma.
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DOI：
10.2147/ott.s124795
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发表时间：
2017
影响因子：
4
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Chen Y;Zhou B;Chen D
膀胱癌是世界上第二大常见的泌尿系恶性肿瘤。在本研究中，我们发现泛素特异性蛋白水解酶(USP21)在BC中表达上调，并且USP21的异位表达与肿瘤大小和转移密切相关。此外，USP21水平高的患者存活率较低。CCK-8、集落形成、伤口愈合和Transwell分析等多种功能分析表明，USP21调控膀胱癌细胞系的增殖和转移。我们还发现USP21可以促进上皮细胞向间充质细胞转化。由于EZH2已被报道促进BC的细胞转移，我们的工作发现USP21去泛素化EZH2并使其稳定。我们的数据表明，USP21可能在调节BC的进展中发挥关键作用，并可能为BC提供潜在的治疗策略。
Bladder cancer (BC) is the second most common malignant tumor of the urinary tract in the world. In this study, we found that ubiquitin-specific protease (USP21) was upregulated in BC and the ectopic expression of USP21 was closely associated with tumor size and metastasis. Moreover, patients with higher levels of USP21 had poorer survival rate. Multiple function analysis such as CCK-8, colony formation, wound healing, and transwell analysis indicated that USP21 regulated cell proliferation and metastasis in bladder carcinoma cell lines. We also found that USP21 could facilitate epithelial–mesenchymal transition. As EZH2 has been reported to promote cell metastasis in BC, our work identified that USP21 deubiquitinated EZH2 and stabilized it. Our data demonstrated that USP21 might play a crucial role in regulating BC progression and could provide a potential therapeutic strategy for BC.