Genome‐scale signatures of adaptive gene expression changes in an invasive seaweed Gracilaria vermiculophylla
Genome‐scale signatures of adaptive gene expression changes in an invasive seaweed Gracilaria vermiculophylla
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DOI：
10.1111/mec.16776
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发表时间：
2022-11
影响因子：
4.9
通讯作者：
Jing Xiang;Saha Mahasweta;Kai-Le Zhong;Q. Zhang;Di Zhang;A. Jueterbock;Stacy A. Krueger‐Hadfield
中科院分区：
文献类型：
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作者：
Jing Xiang;Saha Mahasweta;Kai-Le Zhong;Q. Zhang;Di Zhang;A. Jueterbock;Stacy A. Krueger‐Hadfield
入侵物种可以通过创始人效应和增强对环境压力的耐受性，成功、快速地殖民新的生态位并扩大范围。然而，促进快速适应恶劣环境的基础分子机制（即基因表达变化）仍然知之甚少。红海藻Gracilaria vermiculophylla原产于西北太平洋，但在过去100年里入侵了北美和欧洲沿海栖息地，它提供了一个极好的模型来检验基因表达水平上增强的耐受性是否有助于其入侵成功。我们从日本的原生地以及德尔马瓦半岛（美国东部）和德国的两个非原生地区收集了 G. vermiculophylla。菌体在公共花园中饲养了 4 个月，期间我们进行了比较转录组 (mRNA) 和 microRNA (miRNA) 测序。 mRNA 表达谱鉴定出 59 个基因在天然和非天然菌体之间表达不同。在这些基因中，大多数都涉及代谢途径，包括光合作用、非生物胁迫以及所有四个非本地位点的产物和激素的生物合成。基于 miRNA 的天然/非天然对的靶基因相关性分析表明，一些靶基因通过表观遗传机制受到正向或负向调节。重要的是，这些基因大多与代谢和防御能力相关（例如金属转运蛋白 Nramp5、衰老相关蛋白、细胞壁相关水解酶、ycf68 蛋白和细胞色素 P450 样 TBP）。因此，我们的基因表达结果表明，代谢过程的资源重新分配很可能是导致入侵范围内的 G. vermiculophylla 的广泛持久性和适应的主要机制。因此，这项研究提供了对入侵介导的快速适应的速度和性质的分子洞察。
Invasive species can successfully and rapidly colonize new niches and expand ranges via founder effects and enhanced tolerance towards environmental stresses. However, the underpinning molecular mechanisms (i.e., gene expression changes) facilitating rapid adaptation to harsh environments are still poorly understood. The red seaweed Gracilaria vermiculophylla, which is native to the northwest Pacific but invaded North American and European coastal habitats over the last 100 years, provides an excellent model to examine whether enhanced tolerance at the level of gene expression contributed to its invasion success. We collected G. vermiculophylla from its native range in Japan and from two non‐native regions along the Delmarva Peninsula (Eastern United States) and in Germany. Thalli were reared in a common garden for 4 months at which time we performed comparative transcriptome (mRNA) and microRNA (miRNA) sequencing. MRNA‐expression profiling identified 59 genes that were differently expressed between native and non‐native thalli. Of these genes, most were involved in metabolic pathways, including photosynthesis, abiotic stress, and biosynthesis of products and hormones in all four non‐native sites. MiRNA‐based target‐gene correlation analysis in native/non‐native pairs revealed that some target genes are positively or negatively regulated via epigenetic mechanisms. Importantly, these genes are mostly associated with metabolism and defence capability (e.g., metal transporter Nramp5, senescence‐associated protein, cell wall‐associated hydrolase, ycf68 protein and cytochrome P450‐like TBP). Thus, our gene expression results indicate that resource reallocation to metabolic processes is most likely a predominant mechanism contributing to the range‐wide persistence and adaptation of G. vermiculophylla in the invaded range. This study, therefore, provides molecular insight into the speed and nature of invasion‐mediated rapid adaption.