Fourier Transform Raman Study of Retinal Isomeric Composition and Equilibration in Halorhodopsin
Fourier Transform Raman Study of Retinal Isomeric Composition and Equilibration in Halorhodopsin
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盐视紫红质视网膜异构组成和平衡的傅里叶变换拉曼研究
DOI：
10.1021/jp963346y
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发表时间：
1997
影响因子：
3.3
通讯作者：
J. Lanyi
中科院分区：
文献类型：
--
作者：
L. Zimányi;J. Lanyi
早期结果表明，对盐视紫红质光反应的描述取决于我们能够区分和剖析全反式生色团的光产物与 13-cis,15-syn 生色团的光产物。我们使用傅立叶变换拉曼光谱（一种非扰动方法）来分析盐杆菌和法老嗜盐杆菌的盐视紫红质在不同条件下的视网膜异构状态。结果表明，前一种蛋白质发生了光适应，并且光适应和暗适应形式的两种异构体的数量与早期计算中根据光谱和数学标准赋予它们的权重一致。后一种蛋白质不发生光适应，该蛋白质大部分但不完全含有全反式视网膜。在没有氯离子（这些阴离子泵的运输底物）的情况下，这两种蛋白质都没有光适应，尽管有 13-顺式光循环，但它们都包含全反式...
Earlier results suggested that the description of the photoreaction of halorhodopsin depends on our being able to distinguish and dissect the photoproducts of the all-trans chromophore from those of the 13-cis,15-syn chromophore. We have used FT Raman spectroscopy, a nonperturbing method, to analyze the isomeric states of the retinal under various conditions in halorhodopsins from Halobacterium salinarium and Natronobacterium pharaonis. The results indicate that light adaptation occurs in the former protein, and the amounts of the two isomers in the light-adapted and dark-adapted forms are consistent with the weights given to them, from spectroscopic and mathematical criteria, in the earlier calculations. No light adaptation occurs in the latter protein, which contains mostly, but not entirely, all-trans-retinal. There is no light adaptation in either protein in the absence of chloride, the transported substrate of these anion pumps, and in spite of a 13-cis-like photocycle, they contain both all-trans- a...