Quantification of doxorubicin and metabolites in rat plasma and small volume tissue samples by liquid chromatography/electrospray tandem mass spectroscopy
Quantification of doxorubicin and metabolites in rat plasma and small volume tissue samples by liquid chromatography/electrospray tandem mass spectroscopy
复制标题
DOI：
10.1016/j.jchromb.2004.04.030
复制
发表时间：
2004-09-05
影响因子：
3
通讯作者：
Straubinger, RM
中科院分区：
文献类型：
--
作者：
Arnold, RD;Slack, JE;Straubinger, RM
多柔比星(DXR)是一种广泛用于治疗人类恶性肿瘤的药物，人们正在研究药物释放技术以提高抗肿瘤的选择性和有效性。建立了小体积生物样品中DXR及其主要代谢物的LC-MS/MS鉴定和定量方法。该方法在治疗相关浓度范围内(0.125-10,000 nM)呈线性关系；在脑组织中，定量下限为0.247 nM，灵敏度为1.4pg。定量DXR和检测代谢物形成的能力可能提供对药物的毒性和生物利用度的洞察，这些药物被结合到脂质体等载体中。版权所有(C)2004 Elsevier B.V.
The anthracycline Doxorubicin (DXR) is used widely for the treatment of human malignancies, and drug delivery technologies are under investigation to enhance antitumor selectivity and effectiveness. A liquid chromatography-tandem mass spectroscopy (LC-MS/MS) method was developed to identify and quantify DXR and key metabolites in small-volume biological samples. The assay was linear over the therapeutically relevant concentration range (0.125-10,000 nM); in brain tissue, the lower limit of quantification was 0.247 nM and the sensitivity was 1.4 pg. The ability to quantify DXR and detect metabolite formation may provide insight into the toxicity and bioavailability of drug incorporated into carriers such as liposomes. OF (C) 2004 Elsevier B.V. All rights reserved.