System L-amino acid transporters are differently expressed in rat astrocyte and C6 glioma cells
System L-amino acid transporters are differently expressed in rat astrocyte and C6 glioma cells
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DOI：
10.1016/j.neures.2004.08.003
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发表时间：
2004-12-01
影响因子：
2.9
通讯作者：
Kim, JK
中科院分区：
文献类型：
--
作者：
Kim, DK;Kim, IJ;Kim, JK
L-氨基酸转运体系统是一个主要的营养物质转运系统，负责中性氨基酸（包括几种必需氨基酸）的Na+非依赖性转运。我们比较和研究了大鼠星形胶质细胞培养物和C6胶质瘤细胞中系统L-氨基酸转运蛋白的表达和功能。大鼠星形胶质细胞培养物表达L型氨基酸转运蛋白2（LAT 2）及其亚基4F 2 hc，而L型氨基酸转运蛋白1（LAT 1）在这些细胞中不表达。C6胶质瘤细胞表达LAT 1，但不表达LAT 2。大鼠星形胶质细胞培养物和C6胶质瘤细胞的[C-14] L-亮氨酸摄取是Na+非依赖性的，并且被系统L选择性抑制剂BCH完全抑制。这些结果表明，运输中性氨基酸，包括几种必需氨基酸到大鼠星形胶质细胞培养物和C6胶质瘤细胞的大部分分别由LAT 2和LAT 1介导。因此，大鼠星形胶质细胞培养物和C6胶质瘤细胞是分别用于检查LAT 2和LAT 1的性质的极好工具。此外，在癌细胞中特异性抑制LAT 1可能是抗癌治疗的新理论基础。(C)2004年由Elsevier爱尔兰有限公司和日本神经科学学会出版。
The system L-amino acid transporter is a major nutrient transport system that is responsible for Na+-independent transport of neutral amino acids including several essential amino acids. We have compared and examined the expressions and functions of the system L-amino acid transporters in both rat astrocyte cultures and C6 glioma cells. The rat astrocyte cultures expressed the L-type amino acid transporter 2 (LAT2) with its subunit 4F2hc, whereas the L-type amino acid transporter 1 (LAT1) was not expressed in these cells. The C6 glioma cells expressed LAT1 but not LAT2 with 4F2hc. The [C-14]L-leucine uptakes by the rat astrocyte cultures and C6 glioma cells were Na+-independent and were completely inhibited by the system L selective inhibitor, BCH. These results suggest that the transport of neutral amino acids including several essential amino acids into rat astrocyte cultures and C6 glioma cells are for the most part mediated by LAT2 and LAT1, respectively. Therefore, the rat astrocyte cultures and C6 glioma cells are excellent tools for examining the properties of LAT2 and LAT1, respectively. Moreover, the specific inhibition of LAT1 in cancer cells might be a new rationale for anti-cancer therapy. (C) 2004 Published by Elsevier Ireland Ltd and the Japan Neuroscience Society.