Relaxin in human decidua.
Relaxin in human decidua.
复制标题
人类蜕膜中的松弛素。
DOI：
10.1210/jcem-51-4-939
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
F. Petrucci
中科院分区：
文献类型：
--
作者：
M. Bigazzi;E. Nardi;P. Bruni;F. Petrucci
松弛素（RLX）已从妊娠猪和大鼠的卵巢中纯化，但未从人体组织中纯化。我们的研究表明，组织提取物和在体外培养的人蜕膜的孵育介质含有松弛素生物活性的物质：抑制自发性子宫收缩和耻骨间韧带的伸长。在Sephadex G-50上层析后，生物活性保留在约6000道尔顿摩尔重量的蛋白质级分中。从蜕膜中提取RLX的产量为15- 33.5GPU/g新鲜组织。这开启了在人类物种中分离和纯化RLX的可能性。
Relaxin (RLX) has been purified from the ovary of the pregnant pig and rat but not from human tissues. Our study shows that tissue extracts and incubation media of in vitro culture of human decidua contain a substance with relaxin bioactivities: the inhibition of spontaneous uterine contractions and the elongation of the interpubic ligament. After chromatography on Sephadex G-50 the bioactivity was retained in a protein fraction of approximately 6000 daltons mol wt. The yield of RLX from decidua was 15--33.5 GPU/g fresh tissue. This opens the possibility of the isolation and purification of RLX in the human species.