Expression of vascular endothelial growth factor (VEGF)-C and -D in gastric carcinoma.
Expression of vascular endothelial growth factor (VEGF)-C and -D in gastric carcinoma.
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DOI：
10.1007/s10147-005-0528-3
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发表时间：
2006-02-01
影响因子：
3.3
通讯作者：
Yanaga, Katsuhiko
中科院分区：
文献类型：
--
作者：
Shida, Atsuo;Fujioka, Shuichi;Yanaga, Katsuhiko
背景：血管内皮生长因子C和血管内皮生长因子D都是血管内皮生长因子受体3(VEGFR)-3(Flt-4)和VEGFR-2(KDR/Flk-1)的配体，被认为参与淋巴管的形成。方法：采用逆转录-聚合酶链式反应检测50例胃癌患者手术切除的正常胃粘膜和癌组织中血管内皮生长因子-C、血管内皮生长因子-D和血管内皮生长因子受体-3的表达。结果：血管内皮生长因子-C和血管内皮生长因子-D的表达与胃癌的淋巴侵袭显著相关(P<；0.05)。虽然在大多数病例中，VEGF-C和-D同时表达，但仅有VEGF-C表达与淋巴结转移有关。VEGFR-3的表达与血管内皮生长因子C和血管内皮生长因子D的表达均相关，但与淋巴结转移无关。结论：VEGF-C和VEGF-D均参与了胃癌细胞的淋巴扩散过程，对判断胃癌患者的淋巴侵袭有一定的临床意义。
BACKGROUND: Both vascular endothelial growth factor (VEGF)-C and (VEGF)-D are ligands of VEGF receptor (VEGFR)-3 (Flt-4) and VEGFR-2 (KDR/FLK-1) and are supposed to participate in lymphangiogenesis. The purpose of this study was to clarify the clinical significance of the expression of these factors and to evaluate their relationship with prognosis in patients with gastric carcinoma.METHODS: Fifty pairs of normal mucosa and cancer specimens were obtained from patients who had undergone gastrectomy for primary gastric carcinoma and subjected to reverse transcriptase-polymerase chain reaction for VEGF-C, VEGF-D, and VEGFR-3.RESULTS: Both VEGF-C and VEGF-D mRNA expression significantly correlated with lymphatic invasion (P < 0.05). Although VEGF-C and -D were concomitantly expressed in most cases, only VEGF-C expression was related to lymph node metastasis. VEGFR-3 expression was associated both with VEGF-C and VEGF-D expression, but not with lymph node metastasis. Tumors expressing these mRNAs tended to correlate with poorer prognosis, but the relationships were not statistically significant.CONCLUSION: Our study suggests that both VEGF-C and VEGF-D are involved in lymphatic spreading of gastric cancer cells, which is clinically useful for the evaluation of lymphatic invasion in patients with gastric carcinoma.