Receptor activator NF κB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis
Receptor activator NF κB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis
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DOI：
10.1034/j.1600-0765.2003.00615.x
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发表时间：
2003-08-01
影响因子：
3.5
通讯作者：
Haynes, D
中科院分区：
文献类型：
--
作者：
Crotti, T;Smith, MD;Haynes, D
目标和背景：本研究调查了调节破骨细胞分化的关键介质、核因子KB配体受体激活剂（RANKL）及其天然抑制剂骨保护素（OPG）在牙周炎中的表达。我们的目的是比较牙周炎患者牙槽骨丢失区域附近的肉芽肿组织中RANKL和OPG的水平与非牙周炎患者组织中的RANKL和OPG水平。此外，我们的目的是确定这些组织中表达这些因子的细胞类型，并证明破骨细胞标志物RANK和抗酒石酸酸性磷酸酶（TRAP）在牙周炎中的表达。材料和方法：使用特异性单克隆抗体分析冷冻活检标本，并通过半定量分析和数字图像分析进行评估，以比较RANKL和OPG蛋白表达水平。使用针对不同细胞谱系特异性标志物的抗体对冷冻切片进行双标记，以确定表达这些蛋白质的细胞类型。结果：RANKL蛋白在牙周炎组织中的表达水平明显高于对照组（P < 0.05）。相反，牙周炎组织中OPG蛋白含量明显降低（P < 0.05）。RANKL蛋白与淋巴细胞和巨噬细胞相关。OPG蛋白与两种组织中的内皮细胞相关。许多白细胞表达RANK mRNA和TRAP观察牙周炎tissue.Conclusion：这些破骨细胞分化的关键调节因子的水平的变化可能发挥了重要作用，在牙周炎中看到的骨丢失。
Objectives and background: This study investigated the expression of key mediators that regulate differentiation of osteoclasts, receptor activator of nuclear factor KB ligand (RANKL), and its natural inhibitor, osteoprotegerin (OPG), in periodonitis. We aimed to compare the levels of the RANKL and OPG in the granulomatous tissue adjacent to areas of alveolar bone loss from patients with periodontitis to that present in tissue from patients without periodontitis. In addition, we aimed to determine the types of cells expressing these factors in these tissues and to demonstrate the expression of the osteoclastic markers, RANK and tartrate-resistant acid phosphatase (TRAP), in periodontitis.Materials and methods: Frozen biopsy specimens were analysed using specific monoclonal antibodies and were evaluated by semiquantitative analysis and digital image analysis to compare levels of RANKL and OPG protein expression. Double labelling of frozen sections with antibodies to different cell lineage specific markers was used to determine the types of cells expressing these proteins. In situ hybridization was used to detect cells expressing RANK mRNA.Results: Semiquantitative image analysis demonstrated that significantly higher levels of RANKL protein (P < 0.05) were expressed in the periodontitis tissue. Conversely, OPG protein was significantly lower (P < 0.05) in the periodontitis tissues. RANKL protein was associated with lymphocytes and macrophages. OPG protein was associated with endothelial cells in both tissues. Many leukocytes expressing RANK mRNA and TRAP were observed in periodontitis tissues.Conclusion: The change in the levels of these key regulators of osteoclast differentiation may play a major role in the bone loss seen in periodontitis.