Effect of saliva collection method on the concentration of protein components in saliva
Effect of saliva collection method on the concentration of protein components in saliva
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DOI：
10.2152/jmi.53.140
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发表时间：
2006-02-01
影响因子：
0.7
通讯作者：
Yasuoka, Susumu
中科院分区：
文献类型：
--
作者：
Michishige, Fumiko;Kanno, Kyoko;Yasuoka, Susumu
为了阐明如何收集唾液用于分析老年饮食不佳者的唾液蛋白质，我们比较了吸痰法、吐痰法和拭子法对正常志愿者唾液蛋白质组分产量的影响。用吸痰法、吐痰法和拭子法收集的唾液分别命名为唾液I、II和III，唾液I收集的唾液量约为唾液II和III的2倍。这主要是由于唾液分泌是由吸力本身刺激的。唾液I和II中总蛋白、S-IgA、胰蛋白酶样活性和人气道胰蛋白酶样蛋白酶（HAT）的含量几乎相同，唾液III中显著低于唾液I和II。激肽释放酶活性在唾液I、II和III中几乎相同。唾液I和唾液II中各总蛋白、S-IgA、激肽释放酶活性、胰蛋白酶活性和HAT的浓度均呈显著正相关。这些结果表明，尽管抽吸法对唾液有一定的刺激作用，但通过对唾液的分析可以获得唾液蛋白变化的信息。
In order to clarify how we collect saliva for analyzing salivary protein in aged subjects who can not eat well, we compared the effects of suction, spitting and the swab saliva collection method on the yield of protein components in saliva samples from normal volunteers. The saliva collected by suction, spitting and the swab method were designated as, Saliva I, II and III, respectively.The saliva volume collected by Saliva I was about 2-fold greater than that by of Saliva II and III. This is mainly due to the fact that saliva secretion was stimulated by the suction itself. The content of total protein, S-IgA, trypsin-like activity and human airway trypsin-like protease (HAT) were almost the same in Saliva I and II, and significantly lower in Saliva III than in Saliva I and II. Kallikrein activity was almost the same in Saliva I, II and III. The concentration of each total protein, S-IgA, kallikrein activity, trypsin activity and HAT in Saliva I were significantly positively correlated with that in Saliva II.These results indicate that we can obtain information of change of salivary protein by analyzing saliva collected by suction method, although this method caused the stimulation of saliva to some extent.