DISTRIBUTION OF FOUR POTENTIAL TRANSMITTER AMINO ACIDS IN MONKEY RETINA
DISTRIBUTION OF FOUR POTENTIAL TRANSMITTER AMINO ACIDS IN MONKEY RETINA
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四种潜在递质氨基酸在猴视网膜中的分布
DOI：
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发表时间：
1977
影响因子：
4.7
通讯作者：
Oliver H. Lowry
中科院分区：
文献类型：
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作者：
S. J. Berger;M. L. McDaniel;J. Carter;Oliver H. Lowry
分析了恒河猴视网膜冷冻干燥切片的离散层的四种氨基酸。甘氨酸的峰值水平被发现的边界附近的内核和内部网状层，并在整个这两个层高。在相邻的神经节细胞和外网状层中，水平低于峰值的50%，在其他地方降至非常低的水平。GABA更严格地限制在内部网状层，并在两侧下降到纤维和感光细胞层峰值的10%或更低。谷氨酸和天冬氨酸在神经节细胞层中含量最高。在无脂质干重基础上，天冬氨酸峰值水平约为大脑的两倍。中度高水平的天冬氨酸和谷氨酸被发现在内部网状和纤维层。在其他地方，水平范围为峰值的20%至50%，视神经中的两种氨基酸相对较低。的氨基酸分布是兼容的GABA在无长突细胞和甘氨酸在水平和无长突细胞的发射机功能。谷氨酸和天冬氨酸在米勒纤维、神经节细胞或两者中可能特别高。
Discrete layers from frozen dried sections of Rhesus monkey retina were analyzed for each of four amino acids. Peak levels of glycine were found near the border of the inner nuclear and inner reticular layers, and were high throughout these two layers. The levels were less than 50% of the peak in the adjacent ganglion cells and outer reticular layers and fell to very low levels elsewhere. GABA was much more sharply restricted to the inner reticular layer and fell off on both sides to levels of 10% or less of the peak in the fiber and photoreceptor cell layers. Glutamate and aspartate were highest in the ganglion cell layer. On a lipid‐free dry weight basis the peak aspartate level was about twice that of brain. Moderately high levels of both aspartate and glutamate were found in the inner reticular and fiber layers. Elsewhere the levels ranged from 20 to 50% of the peak, and both amino acids were relatively low in optic nerve. The amino acid distributions are compatible with a transmitter function for GABA in amacrine cells and for glycine in horizontal and amacrine cells. Glutamate and aspartate may be especially high in Müller fibers, ganglion cells or both.