Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.
Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.
复制标题
与剪接因子 spU2AF59 相互作用的新型裂殖酵母基因 spUAP2 的分子表征。
DOI：
10.1007/s002940050284
复制
发表时间：
1997
期刊：
影响因子：
2.5
通讯作者：
Potashkin,J
中科院分区：
文献类型：
--
作者：
McKinney,R;Wentz-Hunter,K;Schmidt,H;Potashkin,J
前体mRNA剪接所必需的蛋白质U2辅助因子（U2 AF）由大小亚基组成。以前，我们克隆和鉴定了两个亚基，spU 2AF 59和spU 2AF 23，从裂殖酵母。我们现在报告一个novelU 2AF相关蛋白，spUAP 2，它与这两个亚基相互作用。SpUAP 2含有一个经典的和一个简并的RNA识别基序（RRM），这两个基序都是与spU 2AF 59相互作用所必需的。相互作用还需要富含精氨酸/丝氨酸的区域和spU 2AF 59的第一个RRM。spUAP 2基因的无效等位基因是致命的。
A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59and spU2AF23, from fission yeast. We now report a novelU2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.