Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking
Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking
复制标题
DOI：
10.1002/jbm.b.32835
复制
发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Elliott, Robert B.
中科院分区：
文献类型：
--
作者：
Hillberg, Anna L.;Kathirgamanathan, Kalyani;Elliott, Robert B.
DIABECELL（R）胶囊包含涂有聚阳离子聚合物聚-L-鸟氨酸（PLO）的藻酸盐（Alg）内核，其被设计为用于加强胶囊壁的稳定剂，其被生物相容性Alg外层掩蔽。这些聚合物微胶囊已在人体临床试验中表现出优异的机械性能和移植后低血糖症的减少;然而，外Alg层的降解使下面的PLO层暴露，这最终导致体内生物相容性降低。在这里，我们的目的是改善胶囊的生物相容性，并增加通过化学交联/改性的PLO层使用京尼平的胶囊表面的亲水性。荧光显微镜证实交联仅限于PLO层。体外实验证实了在一个月的过程中，化学修饰的胶囊内的胰岛活力和胰岛素释放，并且体内研究表明，当将标准Alg/PLO/Alg胶囊与京尼平修饰的胶囊进行比较时，生物相容性得到改善。(c)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2013.
DIABECELL (R) capsules comprise an inner core of alginate (Alg) coated with a polycationic polymer, poly-L-ornithine (PLO), designed as a stabilizing agent for strengthening the capsule wall, which is masked by an outer layer of biocompatible Alg. These polymeric microcapsules have demonstrated excellent mechanical properties and a reduction in hypoglycemia after tranplantation in human clinical trials; however, degradation of the outer Alg layer leaves the underlying layers of PLO exposed, which ultimately leads to reduced biocompatibility in vivo. Here we aim to improve capsule biocompatibility and to increase the hydrophilic properties of the capsule surface through chemical crosslinking/modification of the PLO layer using genipin. Fluorescence microscopy established crosslinking was limited to the layers of PLO. In vitro experiments confirmed islet viability and insulin release within chemically modified capsules over the course of a month and in vivo investigations demonstrated improved biocompatibility when comparing standard Alg/PLO/Alg capsules with genipin modified capsules. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.