A new putative cyclic nucleotide-gated channel gene, cng-3, is critical for thermotolerance in Caenorhabditis elegans
A new putative cyclic nucleotide-gated channel gene, cng-3, is critical for thermotolerance in Caenorhabditis elegans
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DOI：
10.1016/j.bbrc.2004.10.060
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发表时间：
2004-12-10
影响因子：
3.1
通讯作者：
Park, CS
中科院分区：
文献类型：
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作者：
Cho, SW;Choi, KY;Park, CS
由tax-4和tax-2基因编码的环核苷酸门控（CNG）通道是秀丽隐杆线虫化学感受和温度感受所必需的。在这里，我们报告的鉴定和表征的cng-3，一个新的CNG通道基因，发现在线虫。CNG-3含有六个跨膜区和一个环核苷酸结合区，与高等动物的CNG通道和TAX-4具有高度同源性。cng-3在蠕虫发育的早期就有表达，并在包括AFD神经元在内的五种感觉神经元中均有表达。虽然cng-3无效突变体显示正常的趋化性挥发性气味，突变蠕虫表现出受损的耐热性。这些结果表明，CNG-3，CNG通道亚基的新成员，可能在线虫的热应激的感觉或反应中发挥关键作用。(C)2004年爱思唯尔公司All rights reserved.
Cyclic nucleotide-gated (CNG) channels encoded by tax-4 and tax-2 genes are required for chemo- and thermo-sensation in Caenorhabditis elegans. Here we report the identification and the characterization of cng-3, a new CNG channel gene, found in C elegans. CNG-3 contains Six Putative transmembrane regions and a cyclic nucleotide-binding domain that show high homology with CNG channels of higher animals as well as TAX-4. The expression of cng-3 is detected from early stages in worm development and restricted in five sensory neurons of amphid including AFD neuron. While a cng-3 null mutant displays normal chemotaxis to volatile odorants, the mutant worms exhibit impaired thermal tolerance. These results indicate that CNG-3, a new member of CNG channel subunits, may play a critical role in sensation or response of thermal stress in C elegans. (C) 2004 Elsevier Inc. All rights reserved.