The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation
The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation
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DOI：
10.1038/s41477-021-01085-8
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发表时间：
2022-01-13
期刊：
影响因子：
18
通讯作者：
Ye, Xingguo
中科院分区：
文献类型：
--
作者：
Wang, Ke;Shi, Lei;Ye, Xingguo
虽然小麦遗传转化技术在过去的十年中取得了很大的进展(1-3)，但作为小麦遗传转化中影响最大的因素，目前通过转基因整合和基因组编辑方法改良小麦的能力受到了限制。在体外培养过程中应用与再生相关的基因可能有助于提高植株的转化效率(4-11)。在本研究中，我们发现，与其他方法相比，过表达来自WUSCHEL家族的小麦基因TaWOX5显著提高了转化效率，而且对基因型的依赖性较小。TaWOXS在小麦愈伤组织中的表达既不抑制芽的分化，也不抑制根的发育。此外，根据可见的植物表型，相对较宽的旗叶，可以清楚地识别成功转化的转基因小麦植株。应用TaWOXS提高了小麦幼胚的转化和再生。TaWOXS在提高单粒小麦、小黑麦、黑麦、大麦和玉米转化效率方面的应用也显示出良好的效果。
Although great progress has been achieved regarding wheat genetic transformation technology in the past decade(1-3), genotype dependency, the most impactful factor in wheat genetic transformation, currently limits the capacity for wheat improvement by transgenic integration and genome-editing approaches. The application of regeneration-related genes during in vitro culture could potentially contribute to enhancement of plant transformation efficiencya(4-11). In the present study, we found that overexpression of the wheat gene TaWOX5 from the WUSCHEL family dramatically increases transformation efficiency with less genotype dependency than other methods. The expression of TaWOXS in wheat calli prohibited neither shoot differentiation nor root development. Moreover, successfully transformed transgenic wheat plants can clearly be recognized based on a visible botanic phenotype, relatively wider flag leaves. Application of TaWOXS improved wheat immature embryo transformation and regeneration. The use of TaWOXS in improvement of transformation efficiency also showed promising results in Triticum monococcum, triticale, rye, barley and maize.