Estimating genetic variability with restriction endonucleases.
Estimating genetic variability with restriction endonucleases.
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作者：
R. Hudson
被认为是同源DNA片段的样品中的序列变异量的估计。假定数据是通过限制性内切酶消化片段获得的，由此确定样品中切割位点的数量和频率。在不假设任何特定的人口遗传模型的人口演变的情况下，估计，p，多态性的样本中的网站的比例。该估计量非常接近于EWENS，SPIELMAN和HARRIS（1981）用对称WRIGHT-FISHER中性模式导出的估计量。ENGELS（1981）最近也提出了一个相同数量的估计量，他在没有假设特定群体遗传模型的情况下得出了他的估计量。导出了p的抽样方差和ENGELS估计量。发现p的抽样方差小于ENGELS估计量的抽样方差。同时，对有自由重组和无重组的对称WRIGHT-FISHER中性模型，得到了θ的抽样方差，θ的估计（= 4 Nu）.
The estimation of the amount of sequence variation in samples of homologous DNA segments is considered. The data are assumed to have been obtained by restriction endonuclease digestion of the segments, from which the numbers and frequencies of the cleavage sites in the sample are determined. An estimator, p, of the proportion of sites that are polymorphic in the sample is derived without assuming any particular population genetic model for the evolution of the population. The estimator is very close to the EWENS, SPIELMAN and HARRIS (1981) estimator that was derived with the symmetric WRIGHT-FISHER neutral mode. ENGELS (1981) has also recently proposed an estimator of the same quantity, and he arrived at his estimator without assuming a particular population genetic model. The sampling variance of p and ENGELS' estimator are derived. It is found that the sampling variance of p is lower than the sampling variance of ENGELS' estimator. Also, the sampling variance of theta, an estimate of theta (=4Nu) is obtained for the symmetric WRIGHT-FISHER neutral model with free recombination and with no recombination.