DETAILED ANALYSIS OF THE PORTION OF THE HERPES-SIMPLEX VIRUS TYPE-1 GENOME ENCODING GLYCOPROTEIN-C
DETAILED ANALYSIS OF THE PORTION OF THE HERPES-SIMPLEX VIRUS TYPE-1 GENOME ENCODING GLYCOPROTEIN-C
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DOI：
10.1128/jvi.45.2.634-647.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
WAGNER, EK
中科院分区：
文献类型：
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作者：
FRINK, RJ;EISENBERG, R;WAGNER, EK
单纯疱疹病毒1型(HSV-1)的HindIII片段L(0.59~0.65)的右侧三分之一编码一个基因家族，其中一些成员似乎通过剪接联系在一起。在这份通讯中描述的实验中，确定了编码该mRNA家族的DNA的核苷酸序列，并对与该DNA序列相关的mRNA进行了精确定位。主要的信使核糖核酸是非剪接的，由2520个核苷酸区域编码。在5‘端的上游是HSV-1启动子特有的TATA和CAT盒序列。3‘端位于包含标称多聚腺苷信号的区域附近。三个次要物种(分别为2400、2200和1900个碱基)似乎与主要mRNA的5‘’端共享一个非常短的前导序列，然后由从主要未剪接mRNA 5‘端下游约100、400和625个碱基开始的不间断DNA序列编码。这些位置映射在与共识剪接受体序列相当吻合的位置或非常接近的位置。第4个mRNA由主要未剪接mRNA 3‘’端的730个核苷酸序列编码，其5‘’端正好位于可识别的TATA和CAT box序列的下游。显然，mRNA是由其自身的启动子控制的。核苷酸序列数据与信使核糖核酸定位相结合，证实了该区域编码的4个潜在多肽。最大的一个长1569个碱基，定义了一个523个氨基酸的蛋白质，具有糖蛋白的序列特征。用抗HSV-1包膜糖蛋白的多特异性抗体(抗env-1血清)进行体外翻译产物的免疫沉淀，并与正品HSV-1糖蛋白C进行比较，初步鉴定该翻译产物为HSV-1糖蛋白C。
The right third of HindIII fragment L (0.59 to 0.65) of herpes simplex virus type 1 (HSV-1) encodes a family of mRNA some members of which appear to be related by splicing. In the experiments described in this communication, the nucleotide sequence of the DNA encoding this mRNA family was determined and the mRNA associated with this DNA sequence were precisely located. The major mRNA species is unspliced and encoded by a 2520-nucleotide region. Just upstream of the 5'' end are TATA and CAT box sequences characteristic of HSV-1 promoters. The 3'' end maps near a region containing a nominal polyadenylation signal. Three minor species (2400, 2200 and 1900 bases, respectively) appear to share a very short leader sequence with the 5'' end of the major mRNA and are then encoded by uninterrupted DNA sequences beginning about 100, 400 and 625 bases downstream of the 5'' end of the major unspliced mRNA. These positions map at or very near positions which agree reasonably well with consensus splice acceptor sequences. The 4th mRNA is encoded by a contiguous 730 nucleotide sequence at the 3'' end of the major unspliced mRNA and has its 5'' end just downstream of recognizable TATA and CAT box sequences. Evidently, mRNA is controlled by its own promoter. The nucleotide sequence data, in combination with the mRNA localization, demonstrate 4 potential polypeptides encoded by the region. The largest is 1569 bases long and defines a 523 amino acid protein with sequence features characteristic of a glycoprotein. This was confirmed to be HSV-1 glycoprotein C by immune precipitation of the in vitro translation product of the major unspliced mRNA, performed with a polyspecific antibody to HSV-1 envelope glycoproteins (anti-env-1 serum), and by comparison of tryptic peptides of this translation product with those of authentic HSV-1 glycoprotein C. Polypeptides encoded by some of the minor species also were tentatively identified.