Cytokinins increase epicatechin content and fungal decay resistance in avocado fruits
Cytokinins increase epicatechin content and fungal decay resistance in avocado fruits
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DOI：
10.1007/s003440010005
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发表时间：
2001-03-01
影响因子：
4.8
通讯作者：
Prusky, D
中科院分区：
文献类型：
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作者：
Beno-Moualem, D;Vinokur, Y;Prusky, D
用模拟细胞分裂素活性的化合物处理源自鳄梨中果皮（MC-2）或果皮（PC-1）的鳄梨愈伤组织提高了表儿茶素的水平。增加培养基中的噻苯隆（TDZ）（0.9 μ M至1.8 μ M）的细胞分裂素依赖（MC-2）愈伤组织中的中果皮的表儿茶素水平的三倍。TDZ还诱导细胞分裂素非依赖性克隆中果皮（MC-1）中表儿茶素的水平，但该组织中表儿茶素的总体水平比细胞分裂素依赖性愈伤组织中低12倍。鳄梨采前喷雾。富埃特果实TDZ（10 μ M）或苄氨基嘌呤（BAP）（40 μ M）增加活性的黄烷酮-3-羟化酶（F3 H）和二氢黄烷酮还原酶（DFR）在2周，以及表儿茶素和抗炭疽病的水平。收获前处理鳄梨品种，Ettinger的果实与苄基腺嘌呤延迟果实软化和腐烂20%。这表明具有细胞分裂素样活性的化合物刺激鳄梨组织中的类黄酮生物合成途径。这可以导致增强的果实抗性，如由C.胶孢菌
Treatment of avocado callus originating from avocado mesocarp (MC-2) or pericarp (PC-1) with compounds mimicking cytokinin activity enhanced the level of epicatechin. Increasing thidiazuron (TDZ) in the medium (0.9 muM to 1.8 muM) tripled the epicatechin level of the mesocarp in cytokinin-dependent (MC-2) callus. TDZ also induced the level of epicatechin in the cytokinin- independent clone mesocarp (MC-1), but the overall level of epicatechin in this tissue was 12-fold lower than in the cytokinin-dependent callus. Preharvest spray of avocado cv. Fuerte fruits with TDZ (10 muM) or benzylaminopurine (BAP) (40 muM) increased activities of flavanone-3-hydroxylase (F3H) and dihydroflavanone reductase (DFR) at 2 weeks as well as levels of epicatechin and resistance to Colletotrichum gloeosporioides. Preharvest treatments of fruits of avocado cv, Ettinger with benzyl adenine delayed fruit softening and decay by 20%. It is suggested that compounds with cytokinin-like activity stimulate the flavonoid biosynthetic pathway in avocado tissue. This can lead to enhanced fruit resistance as shown by the delay in decay development due to C. gloeosporioides.