A DNA polymorphism influencing α(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion
A DNA polymorphism influencing α(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion
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DOI：
10.1007/s002510000263
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发表时间：
2000-11-01
期刊：
影响因子：
3.2
通讯作者：
Vögeli, P
中科院分区：
文献类型：
--
作者：
Meijerink, E;Neuenschwander, S;Vögeli, P
α(1，2)岩藻糖基转移酶(FUT1和FUT2)有助于血型抗原结构的形成，这些结构存在于细胞膜和分泌物中。在本研究中，我们证明了FUT1和FUT2在猪的小肠中都有表达。FUT1基因多态性影响F18菌毛大肠杆菌(ECF18)与肠粘膜的粘附性，FUT2基因与红细胞抗原0的表达有关。FUT1多态导致103位(Ala--&Gt；Thr)和286位(Arg--&Gt；Glu)的氨基酸替换。严格控制FUT2基因的表达会导致小肠粘膜中mRNA的丰富或缺失。ECF18抗性动物被证明是纯合子的苏氨酸在FUT1酶的103位氨基酸。对ECF18黏附的敏感性似乎完全依赖于肠上皮细胞中FUT1的活性。在表达FUT1而不表达FUT2 RNA的ECF18抗性猪的肠粘膜中，α(1，2)岩藻糖基转移酶活性水平显著降低(28~45倍，P
The alpha (1,2)fucosyltransferases (FUT1 and FUT2) contribute to the formation of blood group antigen structures, which are present on cell membranes and in secretions. In the present study we demonstrate that both FUT1 and FUT2 are expressed in the pig small intestine. FUT1 polymorphisms influence adhesion of F18 fimbriated Escherichia coli (ECF18) to intestinal mucosa, and FUT2 is associated with expression of erythrocyte antigen 0. The FUT1 polymorphisms result in amino acid substitutions at positions 103 (Ala-->Thr) and 286 (Arg-->Glu). Tightly controlled expression of the FUT2 gene results in either an abundance or an absence of mRNA in small intestinal mucosa. ECF18-resistant animals were shown to be homozygous for threonine at amino acid 103 of the FUT1 enzyme. Susceptibility to ECF18 adhesion appeared to be solely dependent on the activity of FUT1 in intestinal epithelia. In intestinal mucosae of ECF18-resistant pigs which expressed FUT1 but not FUT2 RNA, the levels of alpha (1,2)fucosyltransferase activity were significantly lower (28- to 45-fold, P