p53 immunodetection of liquid-based processed urinary samples helps to identify bladder tumours with a higher risk of progression.
p53 immunodetection of liquid-based processed urinary samples helps to identify bladder tumours with a higher risk of progression.
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DOI：
10.1038/sj.bjc.6602684
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发表时间：
2005-07-25
影响因子：
8.8
通讯作者：
Devonec, M
中科院分区：
文献类型：
--
作者：
Piaton, E;Faynel, J;Ruffion, A;Lopez, J;Perrin, P;Devonec, M
p53可以帮助识别具有从浅表性疾病进展为浸润性疾病风险的膀胱肿瘤病例。半自动液基细胞学（LBC）技术为分子技术的发展提供了机会。本研究的目的是调查LBC是否可以改善p53免疫标记，并评估尿p53是否有预后价值。在用Cytyc Thinprep®处理器处理后的198个尿液样品中研究了p53的免疫反应性。抗原修复后，用识别野生型和突变型p53蛋白的单克隆抗体（克隆DO-7，Dako）（1/1000）标记细胞。p53阳性率为17.2%。高级别（G3）肿瘤阳性率为74.1%。相比之下，低级别（G1-2）尿路上皮癌阳性率为23.5%。在中位随访26个月期间，p53过表达的病例中复发率为52.9%，而阴性病例中复发率仅为10.9%（P<0.001）。p53阳性组的进展率为35.3%，p53阴性组为5.5%（P<0.001）。p53蓄积患者的无进展生存期显著缩短（P=0.007）。在分级和分期分层的多变量分析中，p53是总生存率的独立预测因子（P=0.042）。结果表明，使用Thinprep® LBC，p53免疫标记排泄的尿路上皮细胞可以检测到大多数高级别肿瘤，并可能有助于识别复发和进展风险较高的病例。
p53 could help identify bladder tumour cases with a risk of progression from superficial to invasive disease. Semiautomatic, liquid-based cytology (LBC) techniques offer an opportunity to standardise molecular techniques. The aim of our study was to investigate whether LBC could improve p53 immunolabelling, and to assess whether urinary p53 could have a prognostic value. Immunoreactivity for p53 was studied in 198 urine samples after treatment with the Cytyc Thinprep® processor. After antigen retrieval, cells were labelled with a monoclonal antibody that recognises both wild-type and mutant form of the p53 protein (Clone DO-7, Dako), 1/1000. Positivity for p53 was assessed in 17.2% of the cases. High-grade (G3) tumours were positive in 74.1% of the cases. Comparatively, low-grade (G1–2) urothelial carcinomas were positive in 23.5% of the cases. During a median follow-up period of 26 months, recurrence was observed in 52.9% of the cases with p53 overexpression, and in only 10.9% of negative cases (P<0.001). The progression rate was 35.3% of p53-positive cases vs 5.5% of p53-negative cases (P<0.001). Progression-free survival was significantly shorter in patients with p53 accumulation (P=0.007). In a multivariate analysis stratified on grade and stage, p53 was an independent predictor of overall survival (P=0.042). The results show that using Thinprep® LBC, p53 immunolabelling of voided urothelial cells allows most high-grade tumours to be detected and may help identify cases with a higher risk of recurrence and progression.
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影响因子：
4.1
作者：
Dumaz, N;Milne, DM;Meek, DW
通讯作者：
Meek, DW
影响因子：
23.4
作者：
Schmitz-Dräger, BJ;Goebell, PJ;Fradet, Y
通讯作者：
Fradet, Y
影响因子：
6
作者：
Hopman, AHN;Kamps, MAF;Ramaekers, FCS
通讯作者：
Ramaekers, FCS
影响因子：
3.3
作者：
MURPHY, WM
通讯作者：
MURPHY, WM
影响因子：
3.4
作者：
Piaton, E;Hutin, K;Cottier, M
通讯作者：
Cottier, M