High genetic structuring of Tula hantavirus
High genetic structuring of Tula hantavirus
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DOI：
10.1007/s00705-016-2762-6
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Ulrich, Rainer G.
中科院分区：
文献类型：
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作者：
Schmidt, Sabrina;Saxenhofer, Moritz;Ulrich, Rainer G.
图拉病毒(Tula Virus，TULV)是一种与田鼠相关的汉坦病毒，对人类具有低致病性或无致病性。在本研究中，686只普通田鼠(Microtus Arvalis)、249只野外田鼠(Microtus Agrestis)和30只水鼠(Arvicola Spec.)在德国、卢森堡和法国的79个地点采集，用RT-PCR和TULV-Ig G ELISA进行筛查。在43个地点检测到TULV特异性RNA和/或抗体，表明该病毒在研究地区的地理分布广泛。普通田鼠TULV感染率(16.7%)高于田鼠(9.2%)和水田鼠(10.0%)。10个诱捕点的时间序列数据表明，TULV RNA在普通田鼠种群中持续存在长达34个月，尽管通常流行程度较低。系统发育分析表明，TULV序列具有较强的地理遗传结构，且与啮齿动物种类无关，证实普通田鼠为优势宿主，可外溢感染共生田田鼠和水田鼠。TULV的系统发育分支与普通田鼠的进化谱系有普遍的联系，这是通过大地理范围的线粒体DNA序列来评估的，但与接触区的局部尺度差异有关。
Tula virus (TULV) is a vole-associated hantavirus with low or no pathogenicity to humans. In the present study, 686 common voles (Microtus arvalis), 249 field voles (Microtus agrestis) and 30 water voles (Arvicola spec.) were collected at 79 sites in Germany, Luxembourg and France and screened by RT-PCR and TULV-IgG ELISA. TULV-specific RNA and/or antibodies were detected at 43 of the sites, demonstrating a geographically widespread distribution of the virus in the studied area. The TULV prevalence in common voles (16.7 %) was higher than that in field voles (9.2 %) and water voles (10.0 %). Time series data at ten trapping sites showed evidence of a lasting presence of TULV RNA within common vole populations for up to 34 months, although usually at low prevalence. Phylogenetic analysis demonstrated a strong genetic structuring of TULV sequences according to geography and independent of the rodent species, confirming the common vole as the preferential host, with spillover infections to co-occurring field and water voles. TULV phylogenetic clades showed a general association with evolutionary lineages in the common vole as assessed by mitochondrial DNA sequences on a large geographical scale, but with local-scale discrepancies in the contact areas.