Regulation and role of PDGF receptor alpha-subunit expression during embryogenesis.
Regulation and role of PDGF receptor alpha-subunit expression during embryogenesis.
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发表时间：
1992-05
期刊：
影响因子：
4.6
通讯作者：
G. Schatteman;Kathleen Morrison-Graham;A. V. Koppen;J. A. Weston;D. F. Bowen-Pope
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文献类型：
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作者：
G. Schatteman;Kathleen Morrison-Graham;A. V. Koppen;J. A. Weston;D. F. Bowen-Pope
血小板衍生生长因子受体α亚基（PDGFR α）是PDGF受体的一种形式，是PDGF A链结合所必需的。PDGFR α在早期胚胎中的表达首先在中胚层形成时被检测到，并且在后来的发育过程中仍然是许多中胚层衍生物的特征。到9.5天的发育，对于补丁突变（PDGFR α的缺失）纯合的胚胎显示出明显的生长迟缓和中胚层结构的缺陷，导致这些胚胎中超过一半的死亡。在这第一个关键时期存活下来的突变胚胎是可行的，直到一组新的缺陷在大多数结缔组织中变得明显。例如，皮肤缺少真皮，许多器官中的结缔组织成分减少。在此阶段，PDGFR α mRNA的表达也出现在神经嵴衍生的间充质中，晚期胚胎缺陷与中胚层和神经嵴衍生物相关。除神经嵴、透镜和脉络丛外，直到中枢神经系统发育后期才在外胚层衍生物中检测到PDGFR α mRNA。在任何发育阶段的任何胚胎内胚层衍生物中均未检测到表达。这些结果表明，PDGFR α在发育过程中差异表达，这种表达是特定组织发育所必需的。
The platelet-derived growth factor receptor alpha-subunit (PDGFR alpha) is the form of the PDGF receptor that is required for binding of PDGF A-chain. Expression of PDGFR alpha within the early embryo is first detected as the mesoderm forms, and remains characteristic of many mesodermal derivatives during later development. By 9.5 days of development, embryos homozygous for the Patch mutation (a deletion of the PDGFR alpha) display obvious growth retardation and deficiencies in mesodermal structures, resulting in the death of more than half of these embryos. Mutant embryos that survive this first critical period are viable until a new set of defects become apparent in most connective tissues. For example, the skin is missing the dermis and connective tissue components are reduced in many organs. By this stage, expression of PDGFR alpha mRNA is also found in neural crest-derived mesenchyme, and late embryonic defects are associated with both mesodermal and neural crest derivatives. Except for the neural crest, the lens and choroid plexus, PDGFR alpha mRNA is not detected in ectodermal derivatives until late in development in the central nervous system. Expression is not detected in any embryonic endodermal derivative at any stage of development. These results demonstrate that PDGFR alpha is differentially expressed during development and that this expression is necessary for the development of specific tissues.