Short-term docosapentaenoic acid (22:5n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats
Short-term docosapentaenoic acid (22:5n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats
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DOI：
10.1017/s0007114509991334
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发表时间：
2010-01-14
影响因子：
3.6
通讯作者：
Sinclair, Andrew J.
中科院分区：
文献类型：
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作者：
Kaur, Gunveen;Begg, Denovan P.;Sinclair, Andrew J.
目前尚不清楚哺乳动物膳食中n-3二十二碳五烯酸（DPA）的代谢结局。本研究的目的是确定大鼠中膳食DPA转化为DHA和EPA的程度。用4组5周龄雄性SD大鼠，每天灌胃给予DPA、EPA、DHA或油酸各50 mg，连续7 d。在给药期结束时，分析组织中长链PUFA的浓度。DPA补充导致所有组织中DPA浓度的显著增加，其中最大的增加是在脂肪中（5倍），最小的增加是在脑中（1.1倍）。DPA补充剂显着增加了肝脏中DHA的浓度以及肝脏、心脏和骨骼肌中EPA的浓度，这可能是通过逆转录过程实现的。补充EPA显著增加了肝脏、心脏和骨骼肌中EPA和DPA的浓度以及肝脏中DHA的浓度。DHA补充剂提高了所有组织中的DHA水平和肝脏中的EPA水平。脂肪是DPA、EPA和DHA积累的主要组织部位。这些数据表明，在短期研究中，膳食DPA可以在肝脏中转化为DHA，此外，它还可以在肝脏、脂肪、心脏和骨骼肌中部分逆转为EPA。未来的研究应检查DPA在肝脏和心脏等组织中的生理作用。
The metabolic fate of dietary n-3 docosapentaenoic acid (DPA) in mammals is currently unknown. The aim of the present study was to determine the extent of conversion of dietary DPA to DHA and EPA in rats. Four groups of male weanling Sprague-Dawley rats (aged 5 weeks) were given 50mg of DPA, EPA, DHA or oleic acid, daily for 7d by gavage. At the end of the treatment period, the tissues were analysed for concentrations of long-chain PUFA. DPA supplementation led to significant increases in DPA concentration in all tissues, with largest increase being in adipose (5-fold) and smallest increase being in brain (1.1-fold). DPA supplementation significantly increased the concentration of DHA in liver and the concentration of EPA in liver, heart and skeletal muscle, presumably by the process of retroconversion. EPA supplementation significantly increased the concentration of EPA and DPA in liver, heart and skeletal muscle and the DHA concentration in liver. DHA supplementation elevated the DHA levels in all tissues and EPA levels in the liver. Adipose was the main tissue site for accumulation of DPA, EPA and DHA. These data suggest that dietary DPA can be converted to DHA in the liver, in a short-term study, and that in addition it is partly retroconverted to EPA in liver, adipose, heart and skeletal muscle. Future studies should examine the physiological effect of DPA in tissues such as liver and heart.