Haploinsufficiency of poly(ADP-ribose) polymerase-1-mediated poly(ADP-ribosyl)ation for centrosome duplication.
Haploinsufficiency of poly(ADP-ribose) polymerase-1-mediated poly(ADP-ribosyl)ation for centrosome duplication.
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DOI：
10.1016/j.bbrc.2007.05.108
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发表时间：
2007-08
影响因子：
3.1
通讯作者：
M. Kanai;W. Tong;Zhao-Qi Wang;M. Miwa
中科院分区：
文献类型：
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作者：
M. Kanai;W. Tong;Zhao-Qi Wang;M. Miwa
中心体在维持染色体稳定性方面起着至关重要的作用。中心体被称为微管组织中心，在有丝分裂过程中参与纺锤体极的形成，从而确保将正确数量的染色体分配给子细胞。异常的中心体复制可导致中心体扩增和染色体不稳定。我们以前已经表明，聚（ADP-核糖）聚合酶-1（PARP-1）是重要的中心体功能和染色体的稳定性。在这项研究中，我们使用PARP-1+/+、PARP-1+/−和PARP-1−/−原代小鼠胚胎成纤维细胞，发现PARP-1基因剂量水平与PARP活性和体内聚（ADP-核糖基）化水平相关，这可以解释PARP-1单倍不足影响中心体复制和染色体稳定性的机制。我们的研究结果强调，在体内的聚（ADP-核糖基）化水平的正确调节是重要的适当的中心体复制和染色体稳定性的维护。
The centrosome plays a vital role in maintaining chromosomal stability. Known as the microtubule organizing center, the centrosome is involved in the formation of spindle poles during mitosis, which ensures the distribution of the correct number of chromosomes to daughter cells. Aberrant centrosome duplication could cause centrosome amplification and chromosomal instability. We have previously shown that poly(ADP-ribose) polymerase-1 (PARP-1) is important for centrosome function and chromosomal stability. In this study, we used PARP-1+/+, PARP-1+/−and PARP-1−/−primary mouse embryonic fibroblasts and found that the level of PARP-1 gene dosage correlates with PARP activity and the in vivo level of poly(ADP-ribosyl)ation, which could explain the mechanism by which PARP-1 haploinsufficiency affects centrosome duplication and chromosomal stability. Our results emphasize that correct regulation of poly(ADP-ribosyl)ation levels in vivo is important for maintenance of proper centrosome duplication and chromosomal stability.