Preparation of functional nanoparticles of mPEG - b - P ( DMA - co - HA ) copolymers via polymerization-induced thermal self-assembly
Preparation of functional nanoparticles of mPEG - b - P ( DMA - co - HA ) copolymers via polymerization-induced thermal self-assembly
复制标题
聚合诱导热自组装制备mPEG-b-P（DMA-co-HA）共聚物功能纳米颗粒
DOI：
10.1002/pol.20230420
复制
发表时间：
2023
影响因子：
3.4
通讯作者：
Akar I
中科院分区：
文献类型：
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作者：
Akar I
自组装聚合物纳米颗粒在药物递送、催化和生物传感等各种生物学应用中引起了极大的兴趣。在这方面，聚合诱导自组装（比萨）已被广泛探索为比常规自组装方法更有效的技术，因为它可以在一锅中进行并且不需要苛刻的条件。近年来出现了一种称为聚合诱导热自组装（PITSA）的方法，利用温敏性聚合物在临界温度点的固有相变行为原位生成温敏性纳米粒子。然而，适用于PITSA的单体范围狭窄限制了温敏性纳米粒子的设计，因此该方法尚未得到充分的探索。在这项研究中，我们展示了基于双甲基N，N-二甲基丙烯酰胺（DMA）和疏水丙烯酸己酯（HA）单体的温度响应性纳米颗粒的制备。这是特别有趣的，因为这些单体产生非响应性均聚物，但在共聚时显示出热响应性行为。将获得的纳米颗粒交联以使其在室温下表征，并进一步用短合成抗菌肽（WR）3官能化以证明其作为抗菌剂的概念验证潜力。总的来说，这项工作扩展了适用于PITSA的单体库，用于生产温敏纳米颗粒，有助于设计用于治疗目的的新功能纳米颗粒。
Self‐assembled polymeric nanoparticles have been of great interest for various biological applications such as drug delivery, catalysis, and biosensing. In this regard, polymerization‐induced self‐assembly (PISA) has been widely explored as a more efficient technique than conventional self‐assembly methods as it can be conducted in one pot and does not require harsh conditions. Recently, a method known as polymerization‐induced thermal self‐assembly (PITSA) has emerged, exploiting the inherent phase transition behavior of thermoresponsive polymers at a critical temperature point to generate thermoresponsive nanoparticles in situ.However, the narrow range of monomers suitable for PITSA limits the design of diverse thermoresponsive nanoparticles, and therefore this process has not yet been explored to its fullest capacity. In this study, we demonstrate the preparation of thermoresponsive nanoparticles based on hydrophilicN,N‐dimethyl acrylamide (DMA) and hydrophobic hexyl acrylate (HA) monomers. This is particularly interesting as these monomers produce non‐responsive homopolymers but display thermoresponsive behavior when copolymerized. The nanoparticles obtained were crosslinked to enable their characterization at room temperature, and further functionalized with a short synthetic antibacterial peptide (WR)3to demonstrate proof‐of‐concept potential as antimicrobial agents. Overall, this work expands the library of monomers amenable to PITSA for the production of thermoresponsive nanoparticles, contributing to the design of new functional nanoparticles for therapeutic purposes.