The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis.
The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis.
复制标题
DOI：
10.1371/journal.ppat.1006189
复制
发表时间：
2017-02
期刊：
影响因子：
6.7
通讯作者：
Kubo Y
中科院分区：
文献类型：
--
作者：
Kodama S;Ishizuka J;Miyashita I;Ishii T;Nishiuchi T;Miyoshi H;Kubo Y
致病真菌的植物感染涉及通过真菌感应和对植物表面信号响应启动的Appressoria，专门感染结构的分化。尚不清楚植物真菌病原体如何控制感染相关的形态发生对植物来源的信号尚不清楚。在这里，我们表明，与形态发生相关的NDR激酶途径（MOR）的cucumber炭疽病真菌coltotrichum orbiculare在感知植物衍生信号后对于appressorium发育至关重要。通过筛选随机插入突变体，我们确定了MOR元件COPAG1（GYP1中的Perish in-perish in-perish in-pripsence of Gyp1）是植物来源的信号传导途径的关键组成部分。 NDR激酶COCBK1（细胞壁生物合成酶-1）的组成型激活补体COPAG1缺陷。此外，COPAG1缺失受损COCBK1磷酸化，这表明COCAG1通过COCBK1激活起作用。在搜索通过MOR促成appressorium诱导的植物信号，我们发现Cutin单体N-二十二烷核通过分生孢子酯酶从宿主角质层降解，作为appressorium发育的信号分子。全基因组开发过程中全基因组的转录分析表明，MOR是造成植物信号诱导的基因的一部分，在致病性中潜在作用。因此，C。Orbiculare的MOR通过与植物来源的信号通信在调节Appressorium发育和发病机理中具有至关重要的作用。
植物致病真菌引起许多最严重的作物疾病。许多真菌病原体形成了专门的感染结构，以响应植物表面信号而称为Appressoria。基于蛋白激酶的信号通路网络调节多种重要的形态学过程，包括多种真菌病原体中的appressorium发育。但是，植物信号与真菌内细胞内转导之间的精确联系知之甚少。在这里，我们报告了与黄瓜炭疽病真菌colletotrichum orbiculare的appressorium形态发生有关的天然分子和同源信号转导途径的鉴定。我们证明，C。orbiculare的广泛保守的形态发生相关的NDR激酶途径（MOR）调节由植物来源信号触发的感染结构发育，并参与发病机理。 Cutin单体N-OCTADECANAL通过分生孢子酯酶从宿主角质层释放出来，作为通过MOR开发appressorium发育的信号分子。灭活的MOR导致植物信号诱导的基因的下调，包括可能促进感染的真菌分泌的蛋白质。因此，MOR是将植物表面信号与感染相关的形态发生和发病机理连接的关键协调员。虽然先前的报道表明，MOR对于控制其他真菌的细胞极性和分化至关重要，但我们的研究在真菌病原体与宿主植物的相互作用中提供了新的作用。
Plant infection by pathogenic fungi involves the differentiation of appressoria, specialized infection structures, initiated by fungal sensing and responding to plant surface signals. How plant fungal pathogens control infection-related morphogenesis in response to plant-derived signals has been unclear. Here we showed that the morphogenesis-related NDR kinase pathway (MOR) of the cucumber anthracnose fungus Colletotrichum orbiculare is crucial for appressorium development following perception of plant-derived signals. By screening of random insertional mutants, we identified that the MOR element CoPag1 (Perish-in-the-absence-of-GYP1) is a key component of the plant-derived signaling pathway involved in appressorium morphogenesis. Constitutive activation of the NDR kinase CoCbk1 (Cell-wall-biosynthesis-kinase-1) complemented copag1 defects. Furthermore, copag1 deletion impaired CoCbk1 phosphorylation, suggesting that CoPag1 functions via CoCbk1 activation. Searching for the plant signals that contribute to appressorium induction via MOR, we found that the cutin monomer n-octadecanal, degraded from the host cuticle by conidial esterases, functions as a signal molecule for appressorium development. Genome-wide transcriptional profiling during appressorium development revealed that MOR is responsible for the expression of a subset of the plant-signal-induced genes with potential roles in pathogenicity. Thus, MOR of C. orbiculare has crucial roles in regulating appressorium development and pathogenesis by communicating with plant-derived signals. Phytopathogenic fungi cause many of the most serious crop diseases. Many fungal pathogens form specialized infection structures, called appressoria in response to plant surface signals. Networks of protein kinase-based signaling pathways regulate a wide variety of key morphological processes including appressorium development in several fungal pathogens. However, the precise link between plant signals and fungal intracellular transduction is poorly understood. Here, we report on the identification of a native molecule and a cognate signal transduction pathway involved in appressorium morphogenesis of the cucumber anthracnose fungus Colletotrichum orbiculare. We demonstrate that the widely conserved morphogenesis-related NDR kinase pathway (MOR) of C. orbiculare regulates infection structure development triggered by plant-derived signals and involves in pathogenesis. The cutin monomer n-octadecanal released from the host cuticle by conidial esterases functions as the signal molecule for appressorium development via MOR. Inactivating MOR resulted in downregulation of the plant-signal-induced genes including fungal secreted proteins that potentially facilitate infection. Thus, MOR is the crucial coordinator connecting plant surface signals with infection-related morphogenesis and pathogenesis. While previous reports have revealed that MOR is crucial for controlling cell polarity and differentiation in other fungi, our study provides its new role in the interaction of fungal pathogens with host plant.