Shunting of leukocytes in rabbit tenuissimus muscle.
Shunting of leukocytes in rabbit tenuissimus muscle.
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兔细肌中白细胞的分流。
DOI：
10.1152/ajpheart.1989.256.1.h85
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Arfors,KE
中科院分区：
文献类型：
--
作者：
Ley,K;Meyer,JU;Intaglietta,M;Arfors,KE
本文研究了兔筋膜内58条微动脉和毛细血管的白细胞分布，这些微动脉和毛细血管构成了毗邻肌筋膜的8个网络。用荧光视频显微镜(吖啶红)和数字图像处理技术对白细胞进行可视化。横小动脉的白细胞浓度显著升高，达到全身计数的143+/-13%(平均+/-SE)。白细胞沿小动脉树进一步聚集，在最远的分支中达到244+/-16%。可以计算出结缔组织中的白细胞聚集可以导致肌肉毛细血管中的白细胞浓度下降到全身数值的89%。这种分流作用在预防白细胞引起的毛细血管阻塞方面可能很重要。在单个动脉分叉处，白细胞以较高的流速优先进入下游分支(回归系数1.11+/-0.04，n=100)。这种优先分布在数量上足以解释观察到的网络中的白细胞分布。
Leukocyte distribution was studied in 58 arterioles and capillaries constituting eight networks in the fascia adjacent to the rabbit tenuissimus muscle. Fluorescence video microscopy (acridine red) and digital image processing were used to visualize the leukocytes. Leukocyte concentration in the transverse arterioles leaving the muscle proper to irrigate the fascia was significantly elevated to 143 +/- 13% (mean +/- SE) of the systemic count. Leukocytes were found to further accumulate along the arteriolar tree, reaching 244 +/- 16% of systemic in the most remote branches. Leukocyte accumulation in the connective tissue can be calculated to lead to a decrease of leukocyte concentration in muscle capillaries to 89% of the systemic value. This shunting effect may be important in preventing leukocyte-induced capillary obstruction. At individual arteriolar bifurcations, leukocytes were found to preferentially enter the downstream branch with higher flow rate (regression coefficient 1.11 +/- 0.04, n = 100). This preferential distribution is quantitatively sufficient to account for the observed leukocyte distribution in the network.