Selection of plasmid molecules for conjugative transfer and replacement strand synthesis in the donor.
Selection of plasmid molecules for conjugative transfer and replacement strand synthesis in the donor.
复制标题
选择用于供体中接合转移和置换链合成的质粒分子。
DOI：
10.1046/j.1365-2958.2002.03195.x
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发表时间：
2002
影响因子：
3.6
通讯作者：
Meyer,RichardJ
中科院分区：
文献类型：
--
作者：
Parker,Christopher;Meyer,RichardJ
接合转移期间供体细胞中的质粒选择和链置换合成通过涉及在交配前将含有碱基对错配的测试质粒DNA电穿孔到供体细胞中的程序进行检查。从允许质粒营养复制的供体细胞转移质粒的多个拷贝。在不允许营养体复制的条件下，在滞后期后进行进一步的转移。供体中的链置换并不仅仅取决于营养复制的起始机制，这表明还存在缀合特异性机制。第一轮和第二轮转移之间的滞后期反对通过滚环复制在主分子上产生的拷贝的假脱机将多个拷贝转移到受体中。
Plasmid selection and strand replacement synthesis in donor cells during conjugative transfer was examined by a procedure involving electroporation of test plasmid DNA, containing a base pair mismatch, into donor cells prior to mating. Multiple copies of the plasmid were transferred from a donor cell that allowed vegetative replication of the plasmid. Under conditions non‐permissive for vegetative replication, there were further rounds of transfer after a lag period. Strand replacement in the donor did not depend solely on the initiation mechanism for vegetative replication, indicating a conjugation‐specific mechanism was also available. The lag period between first and second rounds of transfer argues against the transfer of multiple copies into recipients by the spooling of copies generated on a master molecule by rolling‐circle replication.