Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration.
Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration.
复制标题
受骨启发的空间特异性压电诱导骨再生
作者：
Yu P;Ning C;Zhang Y;Tan G;Lin Z;Liu S;Wang X;Yang H;Li K;Yi X;Zhu Y;Mao C
骨的细胞外基质可以被描绘为由纤维压电胶原材料和非压电非胶原材料的平行散布域制成的材料。为了模拟这种增强骨再生的特征，构造了由两个平行散布的域（分别具有较高和较低的压电性）制成的材料以形成微尺度压电区（MPZ）。MPZ的生产使用一个通用的和有效的激光照射技术，其中K0.5Na0.5NbO3（KNN）陶瓷选择性地照射，以实现微区相变。激光辐照微区的相结构从正交相和四方相的混合物（具有较高的压电性）改变为四方主导相（具有较低的压电性）。微区压电分布的结果在空间上特定的表面电荷分布，使MPZs承担骨样微尺度电线索。因此，MPZ在体外诱导干细胞的成骨分化，在体内诱导骨再生，即使没有用干细胞接种。模拟骨中的空间特异性压电性的概念将促进未来组织再生材料的合理设计的研究。
The extracellular matrix of bone can be pictured as a material made of parallel interspersed domains of fibrous piezoelectric collagenous materials and non-piezoelectric non-collagenous materials. To mimic this feature for enhanced bone regeneration, a material made of two parallel interspersed domains, with higher and lower piezoelectricity, respectively, is constructed to form microscale piezoelectric zones (MPZs). The MPZs are produced using a versatile and effective laser-irradiation technique in which K0.5Na0.5NbO3 (KNN) ceramics are selectively irradiated to achieve microzone phase transitions. The phase structure of the laser-irradiated microzones is changed from a mixture of orthorhombic and tetragonal phases (with higher piezoelectricity) to a tetragonal dominant phase (with lower piezoelectricity). The microzoned piezoelectricity distribution results in spatially specific surface charge distribution, enabling the MPZs to bear bone-like microscale electric cues. Hence, the MPZs induce osteogenic differentiation of stem cells in vitro and bone regeneration in vivo even without being seeded with stem cells. The concept of mimicking the spatially specific piezoelectricity in bone will facilitate future research on the rational design of tissue regenerative materials.