A transient assay for rapid functional analysis of transcription factors in arabidopsis
A transient assay for rapid functional analysis of transcription factors in arabidopsis
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DOI：
10.1023/a:1007442607645
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发表时间：
1998-06-01
影响因子：
2.1
通讯作者：
Ruberti, I
中科院分区：
文献类型：
--
作者：
Sessa, G;Borello, U;Ruberti, I
我们修改了生物学过程，建立了一种快速分析拟南芥转录因子的瞬时测定方法。拟南芥HD-Zip蛋白ATHB-1在拟南芥叶片颗粒轰击实验中被用作交易因子，并被发现以结合位点特异性的方式反激活一个报告基因。实验结果表明，利用微弹丸介导的基因转移技术快速分析拟南芥转录因子的功能是可行的。
We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.