Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine.
Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine.
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DOI：
10.1016/j.ynpai.2020.100055
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发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Zenke M
中科院分区：
文献类型：
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作者：
Lampert A;Bennett DL;McDermott LA;Neureiter A;Eberhardt E;Winner B;Zenke M
ips细胞衍生模型研究外周感觉神经元的新技术应运而生。遗传疼痛综合征，例如nav通道的功能获得和功能丧失突变是有帮助的。神经性疼痛的个体化治疗可以通过ips细胞衍生的伤害感受器来确定。在这篇简明的迷你综述中，我们将总结在人类干细胞衍生模型中研究外周感觉神经系统生理学和病理生理学的新技术的最新进展。我们将重点介绍体细胞重编程为诱导多能干细胞，神经分化为神经祖细胞和人类感觉神经元的最新进展。我们将总结这种新技术在人类神经病变疾病建模方面的巨大潜力，重点是遗传疼痛综合征，如电压门控钠通道中的功能增益和功能丧失突变。干细胞衍生的人类感觉神经元用于药物测试，我们将总结它们在神经性疼痛患者个体化治疗鉴定中的用途。综述了该技术在伴随诊断和个体化医疗中的潜在应用前景。
New techniques emerge to study peripheral sensory neurons in iPS-cell derived models. Genetic pain syndromes, e.g. gain- and loss-of-function mutations in Nav-channels are helpful. Individualized treatment for neuropathic pain can be identified with iPS-cell derived nociceptors. In this concise Mini-Review we will summarize ongoing developments of new techniques to study physiology and pathophysiology of the peripheral sensory nervous system in human stem cell derived models. We will focus on recent developments of reprogramming somatic cells into induced pluripotent stem cells, neural differentiation towards neuronal progenitors and human sensory neurons. We will sum up the high potential of this new technique for disease modelling of human neuropathies with a focus on genetic pain syndromes, such as gain- and loss-of-function mutations in voltage-gated sodium channels. The stem cell derived human sensory neurons are used for drug testing and we will summarize their usefulness for individualized treatment identification in patients with neuropathic pain. The review will give an outlook on potential application of this technique as companion diagnostics and for personalized medicine.