Evolution of the Protein Corona of Lipid Gene Vectors as a Function of Plasma Concentration
Evolution of the Protein Corona of Lipid Gene Vectors as a Function of Plasma Concentration
复制标题
DOI：
10.1021/la202912f
复制
发表时间：
2011-12-20
期刊：
影响因子：
3.9
通讯作者：
Lagana, Aldo
中科院分区：
文献类型：
--
作者：
Caracciolo, Giulio;Pozzi, Daniela;Lagana, Aldo
在细胞-纳米材料相互作用中感兴趣的有效单元是颗粒及其相关蛋白质的冠的概念正在出现。在这里，我们研究了1，2-二油酰基-3-三甲基铵丙烷（DOTAP）阳离子脂质体（CL）和DOTAP/DNA脂质复合物在宽范围的血浆浓度（2.5-80%）的蛋白质冠的组成演变。脂复合物的硬冠的组成是相当稳定的，但CL的演变相当大。我们发现，CLs的蛋白质冠是由低亲和力和竞争性结合蛋白质，其相对丰度随血浆浓度的变化。这一结果可能具有深刻的生物学意义的应用脂质为基础的基因载体在体外和体内。
The concept that the effective unit of interest in the cell-nanomaterial interaction is the particle and its corona of associated proteins is emerging. Here we investigate the compositional evolution of the protein corona of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) and DOTAP/DNA lipoplexes over a wide range of plasma concentrations (2.5-80%). The composition of the hard corona of lipoplexes is quite stable, but that of CLs does evolve considerably. We show that the protein corona of CLs is made of both low-affinity and competitive-binding proteins whose relative abundance changes with the plasma concentration. This result may have deep biological implications for the application of lipid-based gene vectors both in vitro and in vivo.