Study of the effect of the surface state on the cytocompatibility of a Co-Cr alloy using human osteoblasts and fibroblasts.
Study of the effect of the surface state on the cytocompatibility of a Co-Cr alloy using human osteoblasts and fibroblasts.
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使用人成骨细胞和成纤维细胞研究表面状态对钴铬合金细胞相容性的影响。
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
M. Harmand
中科院分区：
文献类型：
--
作者：
A. Naji;M. Harmand
钴铬基合金广泛应用于口腔和骨科种植。虽然它们的耐受性相对较好，但有时由于合金的生物相容性不足，可能会发生生物并发症。本研究显示了一种合金（Co (base), 28% Cr, 5.5% Mo, 1% Ni, 0.95% Si, 0.7% Fe, 0.65% Mn, 0.25% C）对来自口腔种植部位的分化人细胞，特别是牙槽骨成骨细胞和牙龈成纤维细胞的影响。合金的细胞相容性是通过研究细胞增殖、测定细胞总蛋白和细胞内碱性磷酸酶含量、细胞骨架和细胞形态来确定的。合金以四种不同的表面状态呈现在电池中：粗糙铸造、镜面抛光、微珠喷砂和射频溅射。结果表明，同一种物质的表面状态不同，其对细胞基本功能和特定功能的影响也不同。对于这种合金，我们可以根据其表面状态对其细胞相容性进行分类：微珠喷砂比镜面抛光大得多，比射频溅射大得多，比毛坯铸造大得多。
Cobalt-chromium-based alloys are widely used in oral and orthopedic implantology. Although they are relatively well tolerated, biological complications could occur which sometimes are due to the insufficient biocompatibility of the alloy. This study shows the effects of an alloy (Co (base), 28% Cr, 5.5% Mo, 1% Ni, 0.95% Si, 0.7% Fe, 0.65% Mn, 0.25% C), on differentiated human cells derived from an oral implantation site, specifically alveolar bone osteoblasts and gingival fibroblasts. The cytocompatibility of the alloy is determined by the study of cell proliferation, determination of total cell protein and intracellular alkaline phosphatase contents, cytoskeleton, and cell morphology. The alloy is presented to the cells in four different surface states: rough cast, specular polished, microbead blasted, and RF sputtered. The results demonstrate that the same material has different effects on the basal and specific cellular functions, according to its surface state. For this alloy we can classify its cytocompatibility according to its surface state in such an order: Microbead blasted much greater than specular polished greater than RF sputtered greater than rough cast.
腐蚀离子与人类唾液的结合。
影响因子：
14
作者：
Mueller,HJ
通讯作者：
Mueller,HJ