Comparison of α2 nicotinic acetylcholine receptor subunit mRNA expression in the central nervous system of rats and mice
Comparison of α2 nicotinic acetylcholine receptor subunit mRNA expression in the central nervous system of rats and mice
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DOI：
10.1002/cne.20762
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发表时间：
2005-12-12
影响因子：
2.5
通讯作者：
Sumikawa, K
中科院分区：
文献类型：
--
作者：
Ishii, K;Wong, JK;Sumikawa, K
烟碱乙酰胆碱受体（nAChR）α 2亚基是第一个被克隆的神经元nAChR。然而，在啮齿动物中的α 2 mRNA的分布数据仅存在于少数研究中。因此，我们采用非放射性原位杂交组织化学方法研究了大鼠和小鼠中枢神经系统中α 2 mRNA的表达。我们检测到强烈的杂交信号位于内丛状层的嗅球，脚间核的中脑，腹侧和背侧被盖核，中缝核的脑桥，腹侧部分的延髓网状核，腹角在大鼠和小鼠的脊髓，并在一些浦肯野细胞的大鼠，但不是小鼠的细胞体。中度表达α 2 mRNA的细胞定位于大脑皮质层V和VI、下托、CA 1的oriens层、内侧隔、斜角带复合体、无名质和杏仁核。它们也位于大鼠的几个中脑核中，而在小鼠中，它们在这些区域中很少或不存在。然而，在延髓上部α 2 mRNA的表达在几个大的神经元的延髓网状细胞核和中缝大核的小鼠，而不是大鼠。获得的数据表明，在大鼠和小鼠中存在类似的α 2 mRNA表达模式，除了少数区域，并提供细胞水平分析的基础。
The nicotinic acetylcholine receptor (nAChR) alpha 2 subunit was the first neuronal nAChR to be cloned. However, data for the distribution of alpha 2 mRNA in the rodent exists in only a few studies. Therefore, we investigated the expression of alpha 2 mRNA in the rat and mouse central nervous systems using nonradioactive in situ hybridization histochemistry. We detected strong hybridization signals in cell bodies located in the internal plexiform layer of the olfactory bulb, the interpeduncular nucleus of the midbrain, the ventral and dorsal tegmental nuclei, the median raphe nucleus of the pons, the ventral part of the medullary reticular nucleus, the ventral horn in the spinal cord of both rats and mice, and in a few Purkinje cells of rats, but not of mice. Cells that moderately express alpha 2 mRNA were localized to the cerebral cortex layers V and VI, the subiculum, the oriens layer of CA1, the medial septum, the diagonal band complex, the substantia innominata, and the amygdala of both animals. They were also located in a few midbrain nuclei of rats, whereas in mice they were either few or absent in these areas. However, in the upper medulla oblongata alpha 2 mRNA was expressed in several large neurons of the gigantocellular reticular nucleus and the raphe magnus nucleus of mice, but not of rats. The data obtained show that a similar pattern of alpha 2 mRNA expression exists in both rats and mice, with the exception of a few regions, and provide the basis for cellular level analysis.