Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
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DOI：
10.1186/s12865-016-0160-1
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发表时间：
2016-06-24
期刊：
影响因子：
3
通讯作者：
Kitazawa H
中科院分区：
文献类型：
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作者：
Tsukida K;Takahashi T;Iida H;Kanmani P;Suda Y;Nochi T;Ohwada S;Aso H;Ohkawara S;Makino S;Kano H;Saito T;Villena J;Kitazawa H
免疫生物学詹氏乳杆菌TL 2937调节来自派尔集合淋巴结（PPMP）的猪单核吞噬细胞，并诱导响应于Toll样受体（TLR）-4活化的促炎细胞因子和抗炎细胞因子的差异产生。鉴于吞噬作用在抗原提呈细胞（APC）活化中所起的重要作用，本工作的目的是研究TL 2937与猪PPMP的相互作用，重点是吞噬作用。此外，本研究旨在探讨L.猪血液单核细胞衍生的树突状细胞（MoDC）中的Jensenii TL 2937类似于PPMP中发现的那些，考虑到MoDC不再现粘膜APC的所有功能。研究表明，猪CD 172 a + PPMPs对L. jensenii TL 2937.有趣的是，我们的结果还显示了非免疫调节L的能力降低。plantarum TL 2766被这些免疫细胞吞噬。L.的吞噬作用Jensenii TL 2937对猪PPMP的作用部分依赖于TLR 2。此外，我们证明了TL 2937菌株能够提高未成熟MoDC中IL-1β、IL-12和IL-10的表达，类似于这种免疫生物细菌对PPMP的作用。此外，与PPMP类似，这些免疫调节作用与TL 2937被未成熟MoDC吞噬的较高能力有关。APC中的微生物识别可以通过配体-受体相互作用有效地介导，然后介导吞噬和信号传导。对于免疫生物素株TL 2937，TLR 2在其与猪APC的相互作用中具有部分作用，并且有必要研究其他受体的作用。深入了解免疫生物素L. jensenii TL 2937与猪APC的融合对于成功开发猪宿主的功能性饲料至关重要。这项研究是朝着这个方向迈出的一步。
Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer’s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1β, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.