ELISA FOR TOXOPLASMA ANTIBODY DETECTION - A COMPARISON WITH OTHER SERODIAGNOSTIC TESTS
ELISA FOR TOXOPLASMA ANTIBODY DETECTION - A COMPARISON WITH OTHER SERODIAGNOSTIC TESTS
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DOI：
10.1136/jcp.33.7.640
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发表时间：
1980-01-01
影响因子：
3.4
通讯作者：
ROLLERI, E
中科院分区：
文献类型：
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作者：
BALSARI, A;POLI, G;ROLLERI, E
开发了一种 ELISA（酶联免疫吸附测定）方法，使用抗原包被的聚苯乙烯珠作为固相，并使用抗人 IgG-辣根过氧化物酶缀合物作为酶示踪剂来测量人血清中的弓形虫 IgG 抗体。为了评估 ELISA 的敏感性和特异性，使用传统血清学测试作为参考（染料测试、交叉联免疫分析、被动血凝、间接免疫荧光）进行方法之间的比较。从对组分类的分析中得出了一些考虑因素：ELISA 特异性看起来与参考测试相当，因为所有这些测试分类为阴性的样本都不是 ELISA 阳性，反之亦然；根据各自的类别频率，ELISA 似乎与血凝和免疫荧光具有更好的相关性；染料测试和交叉联免疫测定的阳性数量要低得多，这表明前一种情况达到了更高的灵敏度。
An ELISA [enzyme-linked immunosorbent assay] method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti-human IgG-horseradish peroxidase conjugate as an enzymatic tracer. To assess ELISA sensitivity and specificity, a between methods comparison was made using conventional serological tests as reference (dye-test, crossover-linked immunoassay, passive hemagglutination, indirect immunofluorescence). From an analysis of the group classifications obtained some considerations emerged: the ELISA specificity looks comparable with that of the reference tests as no sample classified as negative by all these tests was ELISA-positive and vice versa; ELISA appears to correlate better with hemagglutination and immunofluorescence on the basis of the respective class frequencies; the number of positives, which is much lower for the dye-test and crossover-linked immunoassay, suggests that a higher sensitivity is reached in the former cases.