Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications
Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications
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DOI：
10.1007/978-1-4939-6352-2_5
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Young, Samuel M., Jr.
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文献类型：
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作者：
Montesinos, Monica S.;Satterfield, Rachel;Young, Samuel M., Jr.
重组病毒载体技术的应用，从基础、临床前和临床水平，对神经科学研究带来了革命性的变化。目前，采用多种重组病毒载体类型，根据所提出的应用，每种载体类型都具有其优点和缺点。辅助病毒依赖性腺病毒载体（HdAd）正在成为理想的病毒载体，其解决了神经科学领域中的主要需求：（1）太大而不能被其他病毒载体包装的转基因的表达和（2）在没有细胞毒性的情况下转基因表达的快速开始。在这里，我们描述了大规模生产HdAd病毒载体的方法，用于体内使用神经特异性转基因表达。
Neuroscience research has been revolutionized by the use of recombinant viral vector technology from the basic, preclinical and clinical levels. Currently, multiple recombinant viral vector types are employed with each having its strengths and weaknesses depending on the proposed application. Helper-dependent adenoviral vectors (HdAd) are emerging as ideal viral vectors that solve a major need in the neuroscience fi eld: (1) expression of transgenes that are too large to be packaged by other viral vectors and (2) rapid onset of transgene expression in the absence of cytotoxicity. Here, we describe the methods for large-scale production of HdAd viral vectors for in vivo use with neurospecifi c transgene expression.