Engineered 3D-printed artificial axons.
Engineered 3D-printed artificial axons.
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DOI：
10.1038/s41598-017-18744-6
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
Van Vliet KJ
中科院分区：
文献类型：
--
作者：
Espinosa-Hoyos D;Jagielska A;Homan KA;Du H;Busbee T;Anderson DG;Fang NX;Lewis JA;Van Vliet KJ
髓鞘形成对于神经元信号的转导、神经元存活和神经系统的正常功能至关重要。髓鞘质疾病是许多使人衰弱的神经系统疾病的原因，例如多发性硬化症和脑白质营养不良。由于缺乏在体外观察和操纵这一过程的实验模型和工具，限制了理解和促进髓鞘形成以及最终开发有效的髓鞘再生疗法的进展。为了解决这个问题，我们开发了神经元轴突的合成模拟物，代表生物轴突的关键几何、机械和表面化学成分。这些人工轴突表现出接近人类轴突的低机械刚度，在无支撑的跨度上有利于神经胶质细胞的接合和包裹，从而能够在反映神经元体内存在的机械线索的环境中研究髓鞘形成。我们的 3D 打印方法能够独立改变人工轴突的复杂特征，从而反映特定的发育、疾病或损伤状态。在这里，我们证明少突胶质细胞的髓鞘质的产生和包裹取决于人工轴突的硬度、直径和配体涂层。该生物仿真平台提供了髓磷脂形成和髓鞘细胞对物理信号和药物的反应的直接可视化和量化。
Myelination is critical for transduction of neuronal signals, neuron survival and normal function of the nervous system. Myelin disorders account for many debilitating neurological diseases such as multiple sclerosis and leukodystrophies. The lack of experimental models and tools to observe and manipulate this process in vitro has constrained progress in understanding and promoting myelination, and ultimately developing effective remyelination therapies. To address this problem, we developed synthetic mimics of neuronal axons, representing key geometric, mechanical, and surface chemistry components of biological axons. These artificial axons exhibit low mechanical stiffness approaching that of a human axon, over unsupported spans that facilitate engagement and wrapping by glial cells, to enable study of myelination in environments reflecting mechanical cues that neurons present in vivo. Our 3D printing approach provides the capacity to vary independently the complex features of the artificial axons that can reflect specific states of development, disease, or injury. Here, we demonstrate that oligodendrocytes’ production and wrapping of myelin depend on artificial axon stiffness, diameter, and ligand coating. This biofidelic platform provides direct visualization and quantification of myelin formation and myelinating cells’ response to both physical cues and pharmacological agents.
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DOI：
10.1016/j.msec.2015.09.102
发表时间：
2016-02-01
影响因子：
7.9
作者：
Baker, Stephen R.;Banerjee, Soham;Guthold, Martin
通讯作者：
Guthold, Martin
影响因子：
3.7
作者：
Jagielska, Anna;Wilhite, Kristen D.;Van Vliet, Krystyn J.
通讯作者：
Van Vliet, Krystyn J.
DOI：
10.1016/j.cub.2015.07.056
发表时间：
2015-09-21
期刊：
Current biology : CB
影响因子：
--
作者：
Bechler ME;Byrne L;Ffrench-Constant C
通讯作者：
Ffrench-Constant C
影响因子：
5.3
作者：
Jagielska A;Lowe AL;Makhija E;Wroblewska L;Guck J;Franklin RJM;Shivashankar GV;Van Vliet KJ
通讯作者：
Van Vliet KJ
影响因子：
2.5
作者：
Lankford, KL;Imaizumi, T;Kocsis, JD
通讯作者：
Kocsis, JD