Effects of UV photofunctionalization on the nanotopography enhanced initial bioactivity of titanium
Effects of UV photofunctionalization on the nanotopography enhanced initial bioactivity of titanium
复制标题
DOI：
10.1016/j.actbio.2011.06.022
复制
发表时间：
2011-10-01
影响因子：
9.7
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Hori, Norio;Iwasa, Fuminori;Ogawa, Takahiro
本研究通过特定的纳米表面特征及其通过UV光功能化的潜在调制来控制钛的生物学能力。将大鼠骨髓来源的成骨细胞培养在单独具有微坑、具有100 nm结节的微坑、具有300 nm结节的微坑或具有500 nm结节的微坑的钛盘上，有或没有UV处理。孵育24小时后，蛋白质吸附，以及成骨细胞的附着，保留和传播进行了检查与钛基板的地形参数。每一个生物事件都是由一组不同的多个表面地形因素控制的，这些因素具有独特的调节模式。例如，在没有UV处理的情况下，钛基底的蛋白质吸附和细胞附着能力随着钛盘的平均粗糙度（Ra）和表面积的增加而线性增加，但是随着纳米颗粒直径的增加而多项式增加。细胞保留能力随着纳米颗粒直径和Ra的增加呈多项式增加，但随着表面积的增加呈线性增加。因此，具有300 nm结节的微坑为这种初始成骨细胞行为和反应创造了最有利的环境。纳米钛表面的UV处理导致所有生物事件的显著增强。然而，紫外线介导的增强模式是不成比例的，指数和压倒性的影响，观察到的生物事件和地形参数。作为一个例子，压倒性的增强，细胞的保留能力，这与各种地形参数的变化波动，变得总是高UV处理后。目前的数据提供了一个基础，了解如何优化纳米结构，以创建钛表面增加的生物学能力，并揭示了一个新的优势，紫外线光功能化的钛基板，协同增加其纳米形貌增强生物学能力，从而大多数的成骨细胞的初始生物学事件是压倒性的增强超出了一个简单的比例增加。(C)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
This study addresses the control of the biological capabilities of titanium through specific nanosurface features and its potential modulation by UV photofunctionalization. Rat bone marrow derived osteoblasts were cultured on titanium disks with micropits alone, micropits with 100 nm nodules, micropits with 300 nm nodules, or micropits with 500 nm nodules, with or without UV treatment. After a 24 h incubation protein adsorption, as well as the attachment, retention, and spread of osteoblasts were examined in correlation with the topographical parameters of the titanium substrates. Each of the biological events was governed by a different set of multiple surface topographical factors with a distinctive pattern of regulation. For instance, without UV treatment the protein adsorption and cell attachment capability of titanium substrates increased linearly with increasing average roughness (Ra) and surface area of titanium disks, but increased polynomially with increasing nanonodule diameter. The cell retention capability increased polynomially with increasing nanonodular diameter and Ra, but increased linearly with increasing surface area. Consequently, the micropits with 300 nm nodules created the most favorable environment for this initial osteoblast behavior and response. UV treatment of the nanonodular titanium surfaces resulted in considerable enhancement of all biological events. However, the pattern of UV-mediated enhancement was disproportionate; exponential and overriding effects were observed depending upon the biological event and topographical parameter. As an example of overriding enhancement, the cell retention capability, which fluctuated with changes in various topographical parameters, became invariably high after UV treatment. The present data provide a basis for understanding how to optimize nanostructures to create titanium surfaces with increased biological capabilities and uncover a novel advantage of UV photofunctionalization of titanium substrates that synergistically increases its nanotopography enhanced biological capabilities whereby most of the initial biological events of osteoblasts were overwhelmingly enhanced beyond a simple proportional increase. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.