Effects of decabrominated diphenyl ether (DBDE) on developmental immunotoxicity in offspring mice
Effects of decabrominated diphenyl ether (DBDE) on developmental immunotoxicity in offspring mice
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DOI：
10.1016/j.etap.2008.06.004
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发表时间：
2008-11-01
影响因子：
4.3
通讯作者：
Kurokawa, Masahiko
中科院分区：
文献类型：
--
作者：
Watanabe, Wataru;Shimizu, Tomomi;Kurokawa, Masahiko
十溴二苯醚（DBDE）是一种广泛存在于环境中的典型溴化阻燃剂，被怀疑对人体有害。我们通过使用呼吸道合胞病毒（RSV）感染后代小鼠的检测系统评估了DBDE的发育免疫毒性。从妊娠第10天到出生后第21天断奶，怀孕小鼠的饮食中持续暴露于十溴二苯醚（百万分之10、100、1000或10，000）。这些母鼠所生的后代小鼠鼻内感染RSV。与对照组相比，在围产期暴露于DBDE的RSV感染后代的肺中病毒滴度呈剂量依赖性增加。在暴露于十溴二苯醚的后代小鼠中，支气管肺泡灌洗液中的干扰素-γ水平和肺部趋化因子RANTES的基因表达也显著升高。组织学分析显示，与对照组相比，接触百万分之10，000的十溴二苯醚的后代小鼠肺部肺炎加重。这些结果表明，DBDE是一种发育免疫毒性剂。(C)2008 Elsevier B. V.保留所有权利。
Decabrominated diphenyl ether (DBDE), a representative brominated flame retardant ubiquitous in the environment, is suspected of being hazardous to humans. We evaluated the developmental immunotoxicity of DBDE by an assay system using respiratory syncytial virus (RSV) infection in offspring mice. Pregnant mice were continuously exposed to DBDE (10, 100, 1000, or 10,000 ppm) in the diet from gestation day 10 to weaning on postnatal day 21. Offspring mice born to these dams were intranasally infected with RSV. Virus titers in the lungs of RSV-infected offspring exposed perinatally to DBDE increased dose-dependently compared with the control. The level of interferon-gamma in the bronchoalveolar lavage fluids and gene expression of the chemokine RANTES in the lungs were also significantly elevated in offspring mice exposed to DBDE. Histopathological analysis revealed that pneumonia in the lungs of offspring mice exposed to 10,000 ppm of DBDE was exacerbated compared with the control. These results indicate that DBDE is a developmental immunotoxic agent. (C) 2008 Elsevier B.V. All rights reserved.