Hydrothermal synthesis of nitrogen and boron co-doped carbon quantum dots for application in acetone and dopamine sensors and multicolor cellular imaging
Hydrothermal synthesis of nitrogen and boron co-doped carbon quantum dots for application in acetone and dopamine sensors and multicolor cellular imaging
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水热合成氮硼共掺杂碳量子点，用于丙酮和多巴胺传感器和多色细胞成像
DOI：
10.1016/j.snb.2018.10.075
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发表时间：
2019-02-15
影响因子：
8.4
通讯作者：
Liu, Shouxin
中科院分区：
文献类型：
--
作者：
Liu, Yushan;Li, Wei;Liu, Shouxin
采用柠檬酸、硼砂和对苯二胺一锅水热法制备了氮硼共掺杂碳量子点（NB-CQDs）。采用透射电子显微镜、傅里叶变换红外光谱、x射线光电子能谱、动态光散射、紫外可见吸收光谱和荧光光谱对合成的NB-CQDs进行表征，表征其表面形貌、晶体结构、官能团、元素组成和光学性质。结果表明，N和B基团被有效地掺杂到点的碳骨架中，在水热反应过程中，NB-CQDs表面形成了含N和含B基团。NB-CQDs的元素掺杂和表面功能化有效地调节了其物理化学性质。NB-CQDs几乎是单分散的，平均粒径为3.53 nm，激发/发射波长分别为360/490 nm。NB-CQDs在不同激发波长下均表现出规律的光致发光（PL），并具有与ph和溶剂极性相关的PL特性。此外，NB-CQD和NB-CQD/聚乙烯醇复合膜对丙酮溶液和蒸汽均有良好的PL响应。设计了丙酮溶液检测限（LOD）为0.54 μ M （R-2 = 0.9956）的PL传感器。NB-CQDs也用于多巴胺的测定。在0.1 ~ 70 μ M范围内，NB-CQDs的PL强度与多巴胺浓度成反比（R-2 = 0.9958）。多巴胺的LOD为11 nM，通过静态猝灭机制发生PL猝灭。此外，这些NB-CQDs可以用于多色细胞成像，并显示出Hepg2细胞的良好摄取。
Nitrogen and boron co-doped carbon quantum dots (NB-CQDs) were prepared via one-pot hydrothermal treatment of citric acid, borax, and p-phenylenediamine.The synthesized NB-CQDs were characterized by transmission electron microscopy, Fourier-transform infrared spectra, X-ray photoelectron spectra, dynamic light scattering, ultraviolet-visible absorption and fluorescence spectra to characterize their surface morphology, crystal structure, functional groups and elemental composition, and optical properties. It was found that N and B species were efficiently doped into the carbon framework of the dots, the N -and B-Containing groups formed on the surface of NB-CQDs during the hydrothermal reaction. The element doping and surface functionalization of NB-CQDs efficiently modulated their physicochemical properties. The NB-CQDs were nearly mono-disperse with an average particle diameter of 3.53 nm, and the excitation/emission wavelengths were 360/490 nm, respectively. The NB-CQDs showed regular photoluminescence (PL) emission under different excitation wavelengths, and possessed pH-dependent and solvent polarity-dependent PL properties. In addition, the NB-CQDs and a NB-CQD/poly(vinyl alcohol) composite film showed good PL responses to acetone solution and vapor. A PL sensor for the determination of acetone solution with a limit of detection (LOD) of 0.54 mu M (R-2 = 0.9956) was then designed. The NB-CQDs were also used for the determination of dopamine. The PL intensity of the NB-CQDs was inversely proportional to the concentration of dopamine in the range of 0.1-70 mu M (R-2 = 0.9958). The LOD of dopamine was 11 nM, and PL quenching occurred via a static quenching mechanism. Additionally, these NB-CQDs can be used for multicolor cellular imaging and showed good uptake by Hepg2 cells.