AUR memorial award--1988. MRI enhancement of perfused tissues using chromium labeled red blood cells as an intravascular contrast agent.
AUR memorial award--1988. MRI enhancement of perfused tissues using chromium labeled red blood cells as an intravascular contrast agent.
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AUR纪念奖--1988年。
DOI：
10.1097/00004424-198910000-00002
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发表时间：
1989
影响因子：
6.7
通讯作者：
JamesJr,AE
中科院分区：
文献类型：
--
作者：
Eisenberg,AD;Conturo,TE;Price,RR;Holburn,GE;Partain,CL;JamesJr,AE
It has been demonstrated that chromium (Cr) labeling significantly decreases the relaxation times of packed red blood cells (RBCs). In this study, the spin-lattice relaxation time (T 1) of human red cells was shortened from 836 ms to 29 ms and the spin-spin relaxation time (T 2) shortened from 134 ms to 18 ms, when the cells were labeled at a Cr incubation concentration of 50 mM. Labeling of canine cells at 50 mM resulted in a T 1 of 36 ms and a T 2 of 26 ms. A labeling concentration of 10 mM produced similar relaxation enhancement, with uptake of 47% of the available Cr, and was determined to be optimal. The enhancement of longitudinal and transverse relaxation rates (1/T 1, 1/T 2) per amount of hemoglobin-bound Cr are 6.9 s− 1 mM− 1 and 9.8 s− 1 mM− 1 respectively, different from those of a pure Cr+ 3 solution. Labeling cells at 10 mM decreased the survival half-time in vivo from 16.6 days to 4.7 days in dogs. No difference in red cell survival was found with the use of hetero-transfusion versus auto-transfusion of labeled RBCs. Significant shortening of the T 1 (912 ms to 266 ms, P=. 03) and T 2 (90 ms to 70 ms, P=. 006) of spleen and the T 1 (764 ms to 282 ms, P=. 005) and the T 2 (128 ms to 86 ms, P=. 005) of liver occurred when 10% of the RBC mass of dogs was exchanged with Cr labeled cells. Liver and spleen spin density changes P> 0.23) and muscle spin density and relaxation changes (P> 0.4) were insignificant. The in vivo T 1 of a canine spleen which had been infarcted did not change following transfusion with labeled cells, where the T 1 of liver did shorten. We believe this preliminary study suggests that Cr labeled red cells may have the potential to become an intravascular magnetic resonance imaging contrast agent.
