REVERSAL OF DIABETES-INSIPIDUS IN BRATTLEBORO RATS - INTRAHYPOTHALAMIC INJECTION OF VASOPRESSIN MESSENGER-RNA
REVERSAL OF DIABETES-INSIPIDUS IN BRATTLEBORO RATS - INTRAHYPOTHALAMIC INJECTION OF VASOPRESSIN MESSENGER-RNA
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DOI：
10.1126/science.1546298
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发表时间：
1992-02-21
期刊：
影响因子：
56.9
通讯作者：
BLOOM, FE
中科院分区：
文献类型：
--
作者：
JIRIKOWSKI, GF;SANNA, PP;BLOOM, FE
信使rna发生在已知分泌催产素和加压素的下丘脑大细胞神经元的轴突内。在Brattleboro大鼠中，有一种基因突变使其不能表达和分泌抗利尿激素，从而导致尿裂症，将从正常大鼠下丘脑纯化的mRNA或合成的抗利尿激素mRNA拷贝注射到下丘脑，导致抗利尿激素选择性摄取、逆行运输和只在大细胞神经元中表达。注射后数小时内可观察到尿崩症的暂时逆转（长达5天）。轴突内mrna可能代表神经元的另一类化学信号。
Messenger RNAs occur within the axons of magnocellular hypothalamic neurons known to secrete oxytocin and vasopressin. In Brattleboro rats, which have a genetic mutation that renders them incapable of vasopressin expression and secretion and thus causes diabetes insipidus, injection into the hypothalamus of purified mRNAs from normal rat hypothalami or of synthetic copies of the vasopressin mRNA leads to selective uptake, retrograde transport, and expression of vasopressin exclusively in the magnocellular neurons. Temporary reversal of their diabetes insipidus (for up to 5 days) can be observed within hours of the injection. Intra-axonal mRNAs may represent an additional category of chemical signals for neurons.