Reusable Dual-Photopolymerized Holographic Glucose Sensors
Reusable Dual-Photopolymerized Holographic Glucose Sensors
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DOI：
10.1002/adfm.202214197
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发表时间：
2023-02-21
影响因子：
19
通讯作者：
Yetisen, Ali K.
中科院分区：
文献类型：
--
作者：
Davies, Samuel;Hu, Yubing;Yetisen, Ali K.
糖尿病是一种广泛的、不可治愈的和致命的疾病，血糖监测和严格的血糖控制对于有效的疾病预防和管理至关重要。基于水凝胶的全息传感器作为一种低成本和无标记的比色传感平台，可通过肉眼和光谱学直接识别，用于定量监测。本文中，通过单脉冲UV诱导的硼酸官能化水凝胶的双光聚合来开发用于护理点（POC）诊断的具有成本效益且可重复使用的全息葡萄糖传感器。全息传感器响应的计算建模进行了以下布拉格定律的制作参数优化和传感器溶胀动力学的研究。研究了全息传感器的制备条件、响应性和干涉性水凝胶组成，以提高响应时间、在尿液中的灵敏度（13.03 nm mmol（-1）L-1）、检测限（0.06 mmol L-1）和可重复使用性。基于水凝胶的全息传感器的光刻图案化允许将附加信息铭刻到传感器中以用于定性测量。在生理葡萄糖浓度范围（0.0-9.4 mmol L-1）内对尿样进行选择性、可逆性和连续监测，以证明糖尿病风险识别的可行性。在可重复使用的尿液分析原型中简单地并入葡萄糖传感器在人尿液中得到验证，表明POC有可能减少患者对侵入性糖尿病监测程序的依赖。
Diabetes is well established as a widespread, incurable, and fatal disease with glucose monitoring and tight glycaemic control vital for effective illness prevention and management. Hydrogel-based holographic sensors serve as a low-cost and label-free colorimetric sensing platform, directly identifiable by the naked eye and spectroscopy for quantitative monitoring. Herein, a cost-effective and reusable holographic glucose sensor is developed via single pulse UV-induced dual-photopolymerization of boronic acid functionalized hydrogels for point-of-care (POC) diagnosis. Computational modeling of holographic sensors response is conducted following Braggs law alongside the study of fabrication parameter optimization and sensor swelling dynamics. Fabrication conditions, responsive and interference hydrogel compositions of holographic sensors are investigated to improve response time, sensitivity in urine (13.03 nm mmol(-1) L-1), limit of detection (0.06 mmol L-1), and reusability. Photolithographic patterning of hydrogel-based holographic sensors permits the inscription of additional information into the sensors for qualitative measurement. Selectivity, reversibility, and continuous monitoring of urine samples are conducted over a physiological glucose concentration range (0.0-9.4 mmol L-1) to demonstrate the viability for diabetic risk identification. The simple incorporation of glucose sensors in a reusable urinary analysis prototype is validated in human urine, showing potential for POC to reduce patient dependency on invasive diabetic monitoring procedures.