miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2
miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2
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DOI：
10.1016/j.canlet.2010.06.012
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发表时间：
2010-12-08
期刊：
影响因子：
9.7
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Ma, Yihui;Yu, Shuangni;Chen, Jie
MicroRNA 是短调节 RNA 越来越多的数据表明改变的 miRNA 参与癌症的发展，并且 miR-27a 在被确定为癌基因的几种类型的癌症中异常上调 尽管在胰腺腺癌中过度表达，但 miR-27a 的致癌作用尚未报道 在这项研究中，我们表明，抑制 miR-27a 抑制了胰腺癌细胞的生长集落形成和迁移 通过使用报告筛选测定，我们发现Sprouty2 (Spty2) 的 3'UTR 携带假定的 miR-27a 结合位点 此外，在胰腺腺癌中具有低表达水平的 Spry2 蛋白通过转染 miR-27a 抑制剂而上调。此处报道的数据首次表明 miR-27a 通过靶向 Spry2 并调节胰腺癌细胞的恶性生物学行为发挥致癌作用，这表明 miR-27a 有可能用作靶点在胰腺癌的诊断和治疗中的应用 (C) 2010 Elsevier Ireland Ltd 版权所有
MicroRNAs are short regulatory RNAs A growing body of data implicates altered miRNA participate in the development of cancers and miR-27a is abnormally upregulated in several types of cancers identified as an oncogene Although overexpressed in pancreatic adenocarcinoma the oncogenic role of miR-27a has not yet been reported In this study we showed that inhibition of miR-27a suppressed the growth colony formation and migration of pancreatic cancer cells By using a reporter-screening assay we discovered that the 3'UTR of Sprouty2 (Spty2) carried a putative miR-27a binding site Furthermore the Spry2 protein which has a low expression level in pancreatic adenocarcinoma was upregulated by transfection with a miR-27a inhibitor The data reported here are the first to indicate that miR-27a plays an oncogenic role by targeting Spry2 and modulating the malignant biological behavior of pancreatic cancer cells This suggests the potential for miR-27a to be used as a target in the diagnosis and treatment of pancreatic adenocarcinoma (C) 2010 Elsevier Ireland Ltd All rights reserved