Microbial diversity in a Pacific Ocean transect from the Arctic to Antarctic circles
Microbial diversity in a Pacific Ocean transect from the Arctic to Antarctic circles
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DOI：
10.3354/ame041091
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发表时间：
2005-11-11
影响因子：
1.4
通讯作者：
Jeffrey, WH
中科院分区：
文献类型：
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作者：
Baldwin, AJ;Moss, JA;Jeffrey, WH
2003年8月下旬至11月上旬，在太平洋北纬70度至南纬68度的一条沿着长约15400 km的样带上，对表层沃茨的微生物多样性进行了研究。比较微生物多样性，确定使用末端限制性片段长度多态性（T-RFLP）分析的PCR扩增的16 S和18 S rDNA限制性内切酶CfoI和MspI消化。细菌数量和叶绿素总量在两个半球的高纬度地区最大，在赤道沃茨有一个较小的峰值。流式细胞术分析表明，原绿球藻在约30 ° N至30 ° S处有一个强峰。每个站点的丰度相对较低，每个样本中有11个原核生物峰，每个群落中有12个真核生物峰。总的来说，原核生物群体似乎更多样化，共产生181个末端限制性片段（T-RF），而真核生物群体共产生135个T-RF。原核生物和真核生物的相似性树状图揭示了4个不同的聚类组，涉及亚北极/北极，温带，热带和亚南极/南极地区。T-RFLP模式表明，微生物群落可能会受到环境水温的影响，中纬度和赤道社区的组成更相似，彼此比冷水社区。原核生物群落的全球分布显示，平均组间相似性为52%，而真核生物群落显示相似性为51%，这意味着太平洋浮游生物群落的组成似乎相当均匀。几个T-RFs无处不在，并作出了重大贡献，每个集群组，而几个T-RFs被观察到是特有的特定的海洋区域。组内相似性> 70%分别归因于原核和真核谱中的12-14个T-RF和8-11个T-RF，表明少数系统发育组负责每个聚类组。
Microbial diversity in surface waters was examined along a similar to 15 400 km transect of the Pacific Ocean from 70 degrees N to 68 degrees S latitude between late August and early November 2003. Comparative microbial diversity was determined using terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S and 18S rDNA restriction digested with CfoI and MspI. Bacterial numbers and total chlorophyll were greatest at higher latitudes in both hemispheres, with a smaller peak in equatorial waters. Flow cytometry analysis indicated a strong peak in Prochlorococcus from approximately 30 degrees N to 30 degrees S. Richness at each station was relatively low, with similar to 11 prokaryotic peaks per sample and similar to 12 eukaryotic peaks per community. Overall, prokaryotic populations appeared more diverse, with 181 total terminal restriction fragments (T-RFs) generated, while eukaryotic populations produced a total of 135 T-RFs. Prokaryotic and eukaryotic similarity dendrograms revealed 4 distinct cluster groups relating to regions sub-Arctic/Arctic, temperate, tropical and sub-Antarctic/Antarctic. T-RFLP patterns suggest that microbial communities may be influenced by ambient water temperature, with mid-latitudinal and equatorial communities more similar in composition to each other than to cold water communities. Global distribution of prokaryotic communities revealed an average inter-group similarity of similar to 52 %, while eukaryotic communities showed similar to 51 % similarity, implying that Pacific planktonic communities appear to be fairly homogenous in composition. Several T-RFs were ubiquitously distributed and contributed significantly to each cluster group, while several T-RFs were observed to be endemic to particular oceanic regions. Within-group similarities of >70 % were attributed to 12-14 T-RFs and 8-11 T-RFs in prokaryotic and eukaryotic profiles, respectively, suggesting that a small number of phylogenetic groups were responsible for each cluster group.