The diversity of retrotransposons in the yeast Cryptococcus neoformans
The diversity of retrotransposons in the yeast Cryptococcus neoformans
复制标题
DOI：
10.1002/yea.733
复制
发表时间：
2001-06-30
期刊：
影响因子：
2.6
通讯作者：
Poulter, RTM
中科院分区：
文献类型：
--
作者：
Goodwin, TJD;Poulter, RTM
我们进行了一项分析的反转录转座子在医学上重要的担子菌真菌隐球菌新生。利用C.在斯坦福大学基因组技术中心的neoforms基因组测序项目中，鉴定了15个不同的LTR反转录转座子家族和几个非LTR反转录转座子家族。至少se，cn家族的成员最近已经转座，并且可能仍然活跃。对于几个科，只有部分元素可以被识别，这些是相当不同的顺序，这表明他们是古老的C。neoformans基因组。大部分C。neoformans元件与先前鉴定的真菌反转录转座子不密切相关，这表明迄今为止真菌反转录转座子的多样性仅被稀疏地取样。C.与白色念珠菌（37个或更多）相比，新型念珠菌具有更少的独特的反转录转座子家族，特别是更少的仅由古老和无活性元件代表的家族，但其具有比酿酒酵母（5个）或粟酒裂殖酵母（2个）多得多的家族。研究结果表明，逆转录转座子的消除在C。neoformans比C. albicans，但可能不如S.酿酒酵母或Sz.粟酒C.反转录转座子的鉴定neoformans应该有助于这一重要病原体的分子特征，也有助于我们进一步了解逆转录因子在基因组进化中所起的作用。版权所有（C）2001约翰威利父子有限公司
We have undertaken an analysis of the retrotransposons in the medically important basidiomycetous fungus Cryptococcus neoformans. Using the data generated by a C. neoformans genome sequencing project at the Stanford Genome Technology Center, 15 distinct families of LTR retrotransposons and several families of non-LTR retrotransposons were identified. Members of at least se, cn families have transposed recently and are probably still active. For several families, only partial elements could be identified and these are quite diverse in sequence, suggesting that they are ancient components of the C. neoformans genome. Most C. neoformans elements are not closely related to previously identified fungal retrotransposons, suggesting that the diversity of fungal retrotransposons has been only sparsely sampled to date. C. neoformans has fewer distinct retrotransposon families than Candida albicans (37 or more), in particular fewer families represented solely by ancient and inactive elements, but it has considerably more families than either Saccharomyces cerevisiae (five) or Schizosaccharomyces pombe (two). The findings suggest that elimination of retrotransposons is faster in C. neoformans than in C. albicans but perhaps not as rapid as in S. cerevisiae or Sz. pombe. The identification of the retrotransposons of C. neoformans should assist in the molecular characterization of this important pathogen, and also further our understanding of the role played by retroelements in genome evolution. Copyright (C) 2001 John Wiley & Sons, Ltd.