Identification of novel split-GAL4 drivers for the characterization of enteroendocrine cells in the Drosophila melanogaster midgut.
Identification of novel split-GAL4 drivers for the characterization of enteroendocrine cells in the Drosophila melanogaster midgut.
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DOI：
10.1093/g3journal/jkac102
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发表时间：
2022-05-30
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影响因子：
--
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果蝇中肠常被作为上皮组织的模型进行研究，原因有很多，其中之一是存在多种称为肠内分泌细胞的分泌细胞。这些细胞的亚群分泌对生物体具有全身作用的肽激素的各种组合。这些激素中的许多也在果蝇的大脑中产生。分裂-GAL 4系统已用于识别和操纵离散的细胞群，但先前表征的分裂-GAL 4驱动程序尚未驱动高比例肠内分泌细胞的表达。在这项研究中，我们使用已知的肠内分泌细胞类型的参考驱动程序筛选了肠内分泌细胞表达的候选分裂GAL 4驱动程序，并发现了一种新的分裂GAL 4驱动程序对，该驱动程序对赋予比先前表征的驱动程序对更多数量的肠内分泌细胞中的表达。新的一对证明大脑表达较少，从而为解开肠道和大脑分泌肽的生理作用提供了更好的工具。我们还鉴定了促进肠内分泌细胞离散亚群表达的其他分裂GAL 4驱动因子。总的来说，这里报道的工具将帮助研究人员更好地靶向肠内分泌细胞亚群。
The Drosophila melanogaster midgut is commonly studied as a model epithelial tissue for many reasons, one of which is the presence of a diverse population of secretory cells called enteroendocrine cells. Subpopulations of these cells secrete various combinations of peptide hormones which have systemic effects on the organism. Many of these hormones are also produced in the Drosophila brain. The split-GAL4 system has been useful for identifying and manipulating discrete groups of cells, but previously characterized split-GAL4 drivers have not driven expression in high proportions of enteroendocrine cells. In this study, we screened candidate split-GAL4 drivers for enteroendocrine cell expression using known reference drivers for this cell type and discovered a new split-GAL4 driver pair that confers expression in a greater number of enteroendocrine cells than previously characterized driver pairs. The new pair demonstrates less brain expression, thereby providing better tools for disentangling the physiological roles of gut- and brain-secreted peptides. We also identified additional split-GAL4 drivers that promote expression in discrete subpopulations of enteroendocrine cells. Overall, the tools reported here will help researchers better target enteroendocrine cell subpopulations.
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影响因子：
14.9
作者：
Larkin A;Marygold SJ;Antonazzo G;Attrill H;Dos Santos G;Garapati PV;Goodman JL;Gramates LS;Millburn G;Strelets VB;Tabone CJ;Thurmond J;FlyBase Consortium
通讯作者：
FlyBase Consortium
影响因子：
3.3
作者：
Dionne H;Hibbard KL;Cavallaro A;Kao JC;Rubin GM
通讯作者：
Rubin GM
DOI：
10.1534/g3.120.401559
发表时间：
2020-11-05
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Miller DE;Kahsai L;Buddika K;Dixon MJ;Kim BY;Calvi BR;Sokol NS;Hawley RS;Cook KR
通讯作者：
Cook KR
影响因子：
3.3
作者：
Ariyapala, Ishara S.;Holsopple, Jessica M.;Sokol, Nicholas S.
通讯作者：
Sokol, Nicholas S.
影响因子：
4.6
作者：
Beehler-Evans, Ryan;Micchelli, Craig A.
通讯作者：
Micchelli, Craig A.