Does Maternal Perinatal Probiotic Supplementation Alter the Intestinal Microbiota of Mother and Child?
Does Maternal Perinatal Probiotic Supplementation Alter the Intestinal Microbiota of Mother and Child?
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DOI：
10.1097/mpg.0000000000000781
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发表时间：
2015-08-01
影响因子：
2.9
通讯作者：
Oien, Torbjorn
中科院分区：
文献类型：
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作者：
Dotterud, Christian K.;Avershina, Ekaterina;Oien, Torbjorn
目的：母体补充益生菌已被证明可以预防后代发生特应性皮炎。我们的目的是调查是否益生菌在怀孕和哺乳期的母亲改变了殖民模式和母亲和儿童的肠道microbiota.Methods的多样性：在一项随机，双盲试验中，妇女接受益生菌牛奶或安慰剂从36周的妊娠期至3个月产后，而母乳喂养。益生菌乳含有鼠李糖乳杆菌GG、嗜酸乳杆菌La-5和动物双歧杆菌亚种。乳酸菌Bb-12。在妊娠30至36周和出生后3个月收集母亲的粪便样本，并在10天，3个月，1岁和2岁时收集儿童的粪便样本，并通过定量聚合酶链反应分析细菌。此外，从3个月大和2岁的儿童粪便样本的特点是使用16 S核糖体RNA基因深度测序，以估计细菌的类和属，和-和-diversity.Results：出生后三个月，无论是患病率和相对丰度的管理益生菌的母亲在益生菌组中相比，在安慰剂组中显着增加。只有鼠李糖乳杆菌GG细菌在10天和3个月大的时候定植在孩子身上。在1岁和2岁的组之间，所施用的益生菌的丰度没有显著差异。对于细菌的类和属，和-和-多样性，有没有组之间的显着差异。结论：不同的益生菌似乎有不同的能力，从母亲转移到孩子。我们没有发现益生菌改变儿童微生物组成或多样性的证据。
Objectives:Maternal probiotic supplementation has been shown to prevent the development of atopic dermatitis in the offspring. We aimed to investigate whether probiotics in pregnant and breast-feeding mothers altered the colonization pattern and the diversity of the mothers' and children's intestinal microbiota.Methods:In a randomized, double-blind trial, women received probiotic milk or placebo from 36 weeks of gestation up to 3 months postnatally while breast-feeding. The probiotic milk contained Lactobacillus rhamnosus GG, L acidophilus La-5, and Bifidobacterium animalis subsp. lactis Bb-12. Stool samples were collected from the mothers at 30 to 36 weeks of gestation and 3 months after birth, and from the child at age 10 days, 3 months, 1 year, and 2 years, and bacteria were analyzed by quantitative polymerase chain reaction. Additionally, stool samples from 3-month-old and 2-year-old children were characterized using 16S ribosomal RNA gene deep sequencing to estimate the bacterial classes and genera, and the - and -diversity.Results:Three months after birth, both the prevalence and the relative abundance of the administered probiotic bacteria were significantly increased among the mothers in the probiotic group compared with among those in the placebo group. Only the Lactobacillus rhamnosus GG bacteria colonized the children at 10 days and at 3 months of age. There were no significant differences in the abundance of the administered probiotic bacteria between the groups at 1 and 2 years of age. For the bacterial classes and genera, and - and -diversity, there were no significant differences between the groups.Conclusions:Different probiotic bacteria seem to have different ability to transfer from the mother to the child. We found no evidence that the probiotics altered the microbial composition or - and -diversity of the children.