Electrochemical molecular analysis without nucleic acid amplification.
Electrochemical molecular analysis without nucleic acid amplification.
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DOI：
10.1016/j.ymeth.2005.05.008
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发表时间：
2005-09
期刊：
影响因子：
4.8
通讯作者：
Haake, DA
中科院分区：
文献类型：
--
作者：
Gau, V;Ma, SC;Wang, H;Tsukuda, J;Kibler, J;Haake, DA
关键词：
电化学生物传感器已经彻底改变了葡萄糖监测，但尚未实现其低成本、直接检测替代基因扩增测试如PCR的承诺[K. Kerman，M.小林、E.田宫，电化学DNA生物传感器技术的最新趋势，Meas。Sci. 15（2004）R1-R11; A. Chaubey，B.D. Malhotra，介导的生物传感器。生物传感器。生物电子。17（6-7）（2002）441-456]。已经预期，纳米级化学结构（例如自组装单分子层）与电化学生物传感器的集成将通过降低固有系统噪声来增加灵敏度。我们设计了一种新的生物传感方法，将这种集成，并实现了快速，超低浓度的灵敏度，而无需目标放大。将原始样品与裂解缓冲液混合，以允许核酸靶与锚和信号探针杂交，然后将信号传导酶固定在生物传感器表面附近。随后施加偏压，并测量环过氧化物酶反应的次级副产物。进一步的研究已经证明了我们的方法在蛋白质，临床化学和离子测定中的应用。
Electrochemical biosensors have revolutionized glucose monitoring but have not yet fulfilled their promise of a low cost, direct detection replacement for genetic amplification tests such as PCR [K. Kerman, M. Kobayashi, E. Tamiya, Recent trends in electrochemical DNA biosensor technology, Meas. Sci. Technol. 15 (2004) R1–R11; A. Chaubey, B.D. Malhotra, Mediated biosensors. Biosens. Bioelectron. 17 (6–7) (2002) 441–456]. It has been anticipated that the integration of nanoscale chemical structures such as self-assembled monolayers with electrochemical biosensors would increase sensitivity by decreasing inherent system noise. We have designed a novel biosensing approach incorporating such integration and achieved rapid, ultra-low concentration sensitivities without target amplification. Raw samples are mixed with lysis buffer to allow hybridization of nucleic acid targets with anchor and signal probes before immobilizing a signaling enzyme proximate to the biosensor surface. A bias potential is subsequently applied and the secondary byproduct of a cyclic peroxidase reaction measured. Further studies have demonstrated the application of our approach in protein, clinical chemistry, and ionic assays.
影响因子：
3.9
作者：
Lahiri, J;Ostuni, E;Whitesides, GM
通讯作者：
Whitesides, GM
影响因子：
3.8
作者：
Sun, CP;Liao, JC;Haake, DA
通讯作者：
Haake, DA
影响因子：
3.9
作者：
Revell, DJ;Knight, JR;Russell, DA
通讯作者：
Russell, DA